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Abstract
<p class="first" id="P1">An ability to visualize HCN in mitochondria in real time
may permit additional insights
into the critical toxicological and physiological roles this classic toxin plays in
living organisms. Herein, we report a mitochondria-specific coumarin pyrrolidinium-derived
fluorescence probe (
<b>MRP1</b>) that permits the real-time ratiometric imaging of HCN in living cells.
The response
is specific, sensitive (detection limit is ca. 65.6 nM), rapid (within 1 s), and reversible.
Probe
<b>MRP1</b> contains a benzyl chloride subunit designed to enhance retention within
the mitochondria
under conditions where the mitochondria membrane potential is eliminated. It has proved
effective in visualizing different concentrations of exogenous HCN in the mitochondria
of HepG2 cells, as well as the imaging of endogenous HCN in the mitochondria of PC12
cells and within neurons. Fluctuations in HCN levels arising from the intracellular
generation of HCN could be readily detected.
</p><p id="P2">
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