Multiplex PCR Sets of Novel Microsatellite Loci for Iwagaki Oyster Crassostrea nippona and Their Application in Parentage Assignment

Abstract

Iwagaki oyster, Crassostrea nippona, widely distributes along the seashore of Eastern Asia, and has been considered to be a potential breeding species due to its delicious taste and high commercial value. In order to study its genetic background and population structure, we developed 46 novel polymorphic microsatellite markers using next-generation sequencing technique and characterized them in 30 individuals. The number of alleles ranged from 3 to 22, while the observed and expected heterozygosities varied from 0.133 to 1.000 and 0.455 to 0.949, respectively. Fifteen microsatellite markers were selected and grouped into five highly informative multiplex PCRs for C. nippona. We evaluated and validated these multiplex PCRs in a cultured population including 173 candidate parents and 486 offspring. In actual parentage analysis, 80% of the offspring were correctly assigned to their parental pairs using three multiplex PCRs. Furthermore, the success rate of parentage assignment reached 96% when the other two multiplex PCRs were added. These 46 microsatellite loci with high variability and the five multiplex PCRs described here provide a powerful tool for pedigree reconstruction, resource conservation and selective breeding program of C. nippona.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 20 December 2019

Publication date (Print): 01 February 2020

Volume: 19

Issue: 1

Pages: 191-198

Affiliations

¹Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China

²Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China

³Institute of Fisheries Science of Guangxi Province, Nanning 530021, China

Author notes

*Corresponding authors: LI Qi, E-mail: qili66@ 123456ouc.edu.cn ; LI Qiongzhen, E-mail: 310687878@ 123456qq.com

Article

Publisher ID: s11802-020-4212-z

DOI: 10.1007/s11802-020-4212-z

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802