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      Differential Expression of Estrogen Receptors Alpha and Beta in the Spinal Cord during Postnatal Development: Localization in Glial Cells

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          Abstract

          Estrogens are recognized as neuroprotective and neurotrophic agents in the central nervous system. They are involved in neuronal differentiation and survival and promote neural development. Estrogen receptors alpha (ER-α) and beta (ER-β) are predominantly expressed in neurons, whereas their presence in glial cells in vivo is more controversial. Changes in their expression during development have been described in different brain areas, but little is known about their presence in the spinal cord. We have carried out an immunohistochemical study in an attempt to analyze the expression of both ERs in astrocytes and oligodendrocytes of the rat spinal cord and their modifications during postnatal development. RT-PCR analysis of whole spinal cord extracts from 4-, 12-, and 25-day-old and adult rats indicated changes in the expression of both receptors during maturation. Immunohistochemistry of slices of the lumbar tract revealed that in an area of the ventral spinal cord that does not contain neuronal cell bodies, but mainly fibers and glial cells, both ER-α and ER-β can be detected. Immunostaining is clearly nuclear, and, in the case of ER-α, both markedly positive and weakly labeled cells can be identified. ER-α is expressed during early development to progressively decline in the adult stage. In contrast, the ER-β signal is low and peaks at postnatal day 25, whereas it is almost undetectable at other ages. Colocalization studies revealed that, at postnatal day 25, ER-α and ER-β are expressed in astrocytes (identified by the specific marker glial fibrillar acidic protein) and oligodendrocytes (labeled by antimyelin 2′,3′-cyclic nucleotide 3′-phosphodiesterase). The present results confirm the expression of ER-α and ER-β in glial cells in vivo and suggest that, also in the spinal cord, glial cells may contribute to the effects of estrogen during development.

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          Most cited references 14

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          Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

          A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
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            Comparative distribution of estrogen receptor-? and -? mRNA in the rat central nervous system

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              Estrogen Actions in the Central Nervous System

               B S McEwen (1999)
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                Author and article information

                Journal
                NEN
                Neuroendocrinology
                10.1159/issn.0028-3835
                Neuroendocrinology
                S. Karger AG
                0028-3835
                1423-0194
                2003
                June 2003
                13 June 2003
                : 77
                : 5
                : 334-340
                Affiliations
                Departments of aExperimental and Clinical Pharmacology, bPhysiological Sciences, and cChemical Sciences, University of Catania, dInstitute of Neurological Sciences, CNR, Section of Catania, and eDepartment of Biochemical, Physiological, and Nutritional Sciences, University of Messina, Italy
                Article
                70899 Neuroendocrinology 2003;77:334–340
                10.1159/000070899
                12806179
                © 2003 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 6, References: 40, Pages: 7
                Categories
                Differentiation of Pituitary Cells

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