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      Transglutaminase inhibition stimulates hematopoiesis and reduces aggressive behavior of crayfish, Pacifastacus leniusculus

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          Abstract

          Transglutaminase (TGase) is a Ca 2+-dependent cross-linking enzyme, which has both enzymatic and nonenzymatic properties. TGase is involved in several cellular activities, including adhesion, migration, survival, apoptosis, and extracellular matrix (ECM) organization. In this study, we focused on the role of the TGase enzyme in controlling hematopoiesis in the crayfish, Pacifastacus leniusculus. We hypothesized that a high TGase activity could mediate an interaction of progenitor cells with the ECM to maintain cells in an undifferentiated stage in the hematopoietic tissue (HPT). We found here that the reversible inhibitor cystamine decreases the enzymatic activity of TGase from crayfish HPT, as well as from guinea pig, in a concentration-dependent manner. Cystamine injection decreased TGase activity in HPT without affecting production of reactive oxygen species. Moreover, the decrease in TGase activity in the HPT increased the number of circulating hemocytes. Interestingly the cystamine-mediated TGase inhibition reduced aggressive behavior and movement in crayfish. In conclusion, we show that cystamine-mediated TGase inhibition directly releases HPT progenitor cells from the HPT into the peripheral circulation in the hemolymph and strongly reduces aggressive behavior in crayfish.

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          Author and article information

          Journal
          J Biol Chem
          J. Biol. Chem
          jbc
          jbc
          JBC
          The Journal of Biological Chemistry
          American Society for Biochemistry and Molecular Biology (11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A. )
          0021-9258
          1083-351X
          11 January 2019
          13 November 2018
          : 294
          : 2
          : 708-715
          Affiliations
          From the []Department of Comparative Physiology, Uppsala University, Norbyvägen 18A, 752 36 Uppsala, Sweden and
          the [§ ]Science for Life Laboratory, Department of Comparative Physiology, Uppsala University, Norbyvägen 18A, 752 36 Uppsala, Sweden
          Author notes
          [1 ] To whom correspondence should be addressed: Science for Life Laboratory, Dept. of Comparative Physiology, Uppsala University, Norbyvägen 18A, 752 36 Uppsala, Sweden. Tel.: 46-18-4712811; Fax: 46-18-4716425; E-mail: Irene.Soderhall@ 123456ebc.uu.se .

          Edited by Luke O'Neill

          Article
          PMC6333896 PMC6333896 6333896 RA118.005489
          10.1074/jbc.RA118.005489
          6333896
          30425101
          9e8a7dfc-191a-4d83-bb11-7f0a3a05007c
          © 2019 Junkunlo et al.

          Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

          History
          : 23 August 2018
          : 4 November 2018
          Funding
          Funded by: Svenska Forskningsrådet Formas (Swedish Research Council Formas) , open-funder-registry 10.13039/501100001862;
          Award ID: 2011–601
          Funded by: Vetenskapsrådet (VR) , open-funder-registry 10.13039/501100004359;
          Award ID: 621–2011-4797
          Award ID: 621–2012-2416
          Categories
          Immunology

          hemolymph,cystamine,hematopoietic stem cells,blood cell,transglutaminase,invertebrate,extracellular matrix,hemocyte,crustacean,arthropod,hematopoiesis

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