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      Glaucoma, Capillaries and Pericytes. 3. Peptide Hormone Binding and Influence on Pericytes

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          Abstract

          To test the potential for vasoactive neuropeptide receptors to affect capillary resistance, we have begun to study the plausibility that pericytes might be equipped to respond to a representative peptide vasoconstrictor and a representative peptide vasodilator. Pericytes cultured from the bovine retinal vasculature specifically bind the angiotensin II (Ang II) antagonist saralasin (1 n M<sup>125</sup>I-saralasin bound at 2.2 ± 0.41 fmol/mg protein) and <sup>125</sup>I-vasoactive intestinal peptide (VIP; K<sub>d</sub> of 0.5 n M with a population of 30 fmol/mg protein). Incubation with 100 µ M Ang II induced minimal cAMP synthesis, while VIP (1 µ M, 10 µ M) did not induce any change in cAMP concentration. Ang II (10 µ M and 100 µ M) caused contraction of pericytes cultured on an elastic silicone surface. Circulating or locally produced vasoactive neuropeptides might affect pericyte contractile tone via several intracellular pathways, moderated by indirect effects of these peptides through endothelial stimulation, with the net effect on local blood flow resulting from the effects on arteries and veins as well as capillaries.

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          Author and article information

          Journal
          OPH
          Ophthalmologica
          10.1159/issn.0030-3755
          Ophthalmologica
          S. Karger AG
          978-3-8055-6378-9
          978-3-318-02122-6
          0030-3755
          1423-0267
          1996
          1996
          01 April 2010
          : 210
          : 5
          : 269-275
          Affiliations
          Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Fla., USA
          Article
          310724 Ophthalmologica 1996;210:269–275
          10.1159/000310724
          8878209
          © 1996 S. Karger AG, Basel

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          Pages: 7
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