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      Three new species of Krogia (Ramalinaceae, lichenised Ascomycota) from the Paleotropics

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          Abstract

          Abstract

          Krogia borneensis Kistenich & Timdal, K. isidiata Kistenich & Timdal and K. macrophylla Kistenich & Timdal are described as new species, the first from Borneo and the two latter from New Caledonia. The new species are supported by morphology, secondary chemistry and DNA sequence data. Krogia borneensis and K. isidiata contain sekikaic and homosekikaic acid, both compounds reported here for the first time from the genus. Krogia macrophylla contains an unknown compound apparently related to boninic acid as the major compound. DNA sequences (mtSSU and nrITS) are provided for the first time for Krogia and a phylogeny of the genus based on 15 accessions of five of the six accepted species is presented. Krogia antillarum is reported as new to Brazil, Guatemala and Mexico.

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          Most cited references17

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          AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS

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            Pcr Primers for the Amplification of Mitochondrial Small Subunit Ribosomal DNA of Lichen-forming Ascomycetes

            Four primers for the amplification of mitochondrial DNA of lichenforming ascomycetes are presented. The primers match the conserved regions U2, U4, and U6, respectively, of mitochondrial small subunit (SSU) ribosomal DNA (rDNA). Polymerase chain reaction using different combinations of the primers produced single amplification products from DNA of eight lichen-forming fungal species but did not amplify DNA of two axenic cultured algal species. The amplification product obtained from Lobaria pulmonaria was sequenced and the 894-bp sequence was compared with the mitochondrial SSU rDNA sequence of Podospora anserine . The two sequences revealed more than 76% identity in the conserved regions U3 to U5 demonstrating that we amplified mitochondrial DNA. The primers matching U2 and U6 yielded amplification products of 800–1000 bp depending on the species examined. The variation observed suggests that mitochondrial SSU rDNA may be useful for phylogenetic analyses of lichen-forming ascomycetes.
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              Improved conditions and new data for identification of lichen products by standardized thin-layer chromatographic method

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                Author and article information

                Contributors
                Journal
                MycoKeys
                MycoKeys
                MycoKeys
                MycoKeys
                Pensoft Publishers
                1314-4057
                1314-4049
                2018
                26 September 2018
                : 40
                : 69-88
                Affiliations
                [1 ] Natural History Museum, University of Oslo, 0318 Oslo, Norway
                [2 ] Finnish Museum of Natural History, University of Helsinki, 00014 Helsinki, Finland
                [3 ] Department of Biosciences, University of Helsinki, 00014 Finland
                [4 ] Department of Life Sciences, The Natural History Museum, London SW75BD, UK
                [5 ] State Museum of Natural History Stuttgart, Rosenstein 1, 70191 Stuttgart, Germany (current address)
                [6 ] Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia
                Author notes
                Corresponding author: S. Kistenich ( s.d.kistenich@ 123456nhm.uio.no )

                Academic editor: Imke Schmitt

                Author information
                https://orcid.org/0000-0001-5501-3210
                Article
                10.3897/mycokeys.40.26025
                6170524
                9f28ca55-98a9-4a35-9d42-9e2ecfcd9126
                Sonja Kistenich, Jouko K. Rikkinen, Holger Thüs, Charles S. Vairappan, Patricia A. Wolseley, Einar Timdal

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 20 April 2018
                : 10 September 2018
                Categories
                Research Article
                Lecanorales
                Lecanoromycetes
                Ramalinaceae
                Identification Key
                Molecular Systematics
                Taxonomy
                Asia
                Australasia
                Malaysia
                Pacific Ocean

                borneo,new caledonia,lichens, phyllopsora ,phylogeny,rainforest,tlc,fungi,lecanorales,ramalinaceae

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