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      Use of Athymic Nude Mice for in vivo Studies of Human Growth-Hormone-Secreting Pituitary Adenomas

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          Abstract

          Human growth hormone (hGH)-secreting pituitary adenoma tissue of 31 acromegalic patients was transplanted subcutaneously onto 291 athymic nude mice. 37% of the transplanted adenoma fragments could be maintained vital up to 46 days. Histological examinations of the transplants revealed neither alterations in their morphological characteristics nor signs of growth. A maintenance or linear decline of hGH secretion of the transplants related to their vitality was observed by hGH radioimmunoassay. Estimation of graft vitality was improved by GH-releasing hormone (GHRH) stimulation in regular intervals. The rate of pituitary adenomas responding to GHRH was as high as in a major collective of acromegalic patients. Our method of positive selection of vital xenotransplanted hGH-secreting pituitary adenomas via hGH detection at regular intervals in combination with GHRH stimulation gives the opportunity of reliable in vivo research with these tumors.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1991
          1991
          02 December 2008
          : 35
          : 5
          : 198-204
          Affiliations
          aDepartment of Neurosurgery, University of Hamburg, and bDepartment of Pathology, Marienkrankenhaus, Hamburg, FRG
          Article
          181902 Horm Res 1991;35:198–204
          10.1159/000181902
          1802823
          © 1991 S. Karger AG, Basel

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          Page count
          Pages: 7
          Categories
          Original Paper

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