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      Molecular cloning and relative tissue expression of keratocan and mimecan in embryonic quail cornea.

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          Abstract

          We have cloned and sequenced the cDNAs for quail cornea keratan sulfate proteoglycan core proteins, keratocan and mimecan. The deduced quail keratocan protein contains a single conservative amino acid difference from the chick sequence, whereas quail mimecan protein contains a 58 amino acid-long avian-unique sequence that shares no homology with mammalian mimecan. Ribonuclease protection assay of Day 16 embryonic quail tissues reveals that keratocan and lumican are expressed at highest levels in cornea, whereas mimecan mRNA is expressed at a much lower level. Keratocan is expressed only in quail cornea, whereas mimecan is expressed in many different tissues as four transcripts of different sizes. Both lumican and mimecan are expressed at lowest levels in brain, liver and sternum.

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          Author and article information

          Journal
          Matrix Biol.
          Matrix biology : journal of the International Society for Matrix Biology
          0945-053X
          0945-053X
          Dec 2000
          : 19
          : 7
          Affiliations
          [1 ] Division of Biology, Kansas State University, Manhattan, KS 66506-4901, USA.
          Article
          S0945-053X(00)00116-5
          11102758
          9f7e540d-5a44-4715-b5cc-810249085ad4
          History

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