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      Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1

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      Journal of Virological Methods
      Elsevier BV

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          Abstract

          Porcine parvovirus (PPV) infection is associated with reproductive losses in swine and its causative agent, the PPV, has been isolated worldwide. Serological surveys and virus isolation studies throughout Brazil confirm the occurrence of PPV infection in this country. The most common methods to detect PPV infection are fluorescent antibody staining of fetal tissues, hemagglutination assay of tissue extracts and virus isolation from fetal tissues. Non-specificity and low sensitivity are the major drawbacks of these techniques. The development of a polymerase chain reaction (PCR) and nested-PCR assays for PPV DNA detection from infected cell lines and clinical samples is described. The primers were designed to a highly conserved region of the PPV genome which codes for the non-structural protein, NS-1. Results showed that PCR could detect PPV in titres at least 10(6) higher than the hemagglutination assay. The PCR and nested-PCR assays were used to detect successfully PPV DNA in clinical samples.

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          Author and article information

          Journal
          Journal of Virological Methods
          Journal of Virological Methods
          Elsevier BV
          01660934
          March 1999
          March 1999
          : 78
          : 1-2
          : 191-198
          Article
          10.1016/S0166-0934(98)00177-3
          10204709
          9ff44da6-444a-413a-83fb-5b8cfd4f2929
          © 1999

          https://www.elsevier.com/tdm/userlicense/1.0/

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