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      Molecular phylogeny and morphology reveal a new epiphytic species of Habenaria (Orchidaceae; Orchideae; Orchidinae) from Nepal

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      PLoS ONE
      Public Library of Science

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          Abstract

          Habenaria is almost cosmopolitan in distribution having predominantly terrestrial orchids, however; a remarkable epiphytic species with some unique morphological characters was collected from Nepal. We conducted a molecular phylogeny of this unusual Habenaria species using nuclear (ITS) and chloroplast ( matK, rbcl) DNA sequence regions to infer its systematic position. Our molecular analyses and morphological treatment recognized this newly collected plant as an undescribed species. This species is described here which is closely related to Habenaria plurifoliata but can be distinguished by having its multiple growing callus-shaped tuber, smaller stature with short stem, longer and wider oblanceolate leaves, peduncle with a foliaceous bract and only one sterile bract, lateral sepals oblong, obtuse, petal apex obtuse, lateral lobes of lip spreading upwards, spur not exceeding the ovary and pedicel.

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          Most cited references22

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          TESTING SIGNIFICANCE OF INCONGRUENCE

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            A rapid DNA isolation procedure from small quantities of fresh leaf tissues

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              Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidaceae)

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                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: MethodologyRole: Writing – original draftRole: Writing – review & editing
                Role: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                23 October 2019
                2019
                : 14
                : 10
                : e0223355
                Affiliations
                [1 ] Nature Research and Conservation Initiatives, Pokharathok, Arghakhanchi, Nepal
                [2 ] Nepal Bioscience Research Laboratory, Banasthali, Kathmandu, Nepal
                University of Sao Paulo, BRAZIL
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                http://orcid.org/0000-0002-9064-8657
                Article
                PONE-D-19-13196
                10.1371/journal.pone.0223355
                6808328
                31644547
                a053daae-032f-4e9b-95f4-9a35677e64c0
                © 2019 Raskoti, Ale

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 9 May 2019
                : 16 September 2019
                Page count
                Figures: 3, Tables: 0, Pages: 12
                Funding
                Funded by: Funding is not available for this research.
                Award ID: Funding is not available for this research.
                The author(s) received no specific funding for this work.
                Categories
                Research Article
                Biology and Life Sciences
                Evolutionary Biology
                Evolutionary Systematics
                Phylogenetics
                Phylogenetic Analysis
                Biology and Life Sciences
                Taxonomy
                Evolutionary Systematics
                Phylogenetics
                Phylogenetic Analysis
                Computer and Information Sciences
                Data Management
                Taxonomy
                Evolutionary Systematics
                Phylogenetics
                Phylogenetic Analysis
                Biology and Life Sciences
                Evolutionary Biology
                Evolutionary Systematics
                Phylogenetics
                Biology and Life Sciences
                Taxonomy
                Evolutionary Systematics
                Phylogenetics
                Computer and Information Sciences
                Data Management
                Taxonomy
                Evolutionary Systematics
                Phylogenetics
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Flower Anatomy
                Pistils
                Plant Ovary
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Flower Anatomy
                Sepals
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Tubers
                Biology and Life Sciences
                Cell Biology
                Cellular Structures and Organelles
                Chloroplasts
                Biology and Life Sciences
                Cell Biology
                Plant Cell Biology
                Chloroplasts
                Biology and Life Sciences
                Plant Science
                Plant Cell Biology
                Chloroplasts
                Biology and Life Sciences
                Cell Biology
                Cellular Types
                Plant Cells
                Chloroplasts
                Biology and Life Sciences
                Cell Biology
                Plant Cell Biology
                Plant Cells
                Chloroplasts
                Biology and Life Sciences
                Plant Science
                Plant Cell Biology
                Plant Cells
                Chloroplasts
                Biology and Life Sciences
                Taxonomy
                New Species Reports
                Computer and Information Sciences
                Data Management
                Taxonomy
                New Species Reports
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Flower Anatomy
                Petals
                Custom metadata
                A list of sampled species, GenBank accession numbers for all sequences and voucher information are provided in S1 Appendix. All other relevant data are within the manuscript and its Supporting Information files.

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