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      Gene-targeted molecular phylogeny, phytochemical profiling, and antioxidant activity of nine species belonging to family Cactaceae


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          Cactaceae plant family comprises over 130 genera and 2000 species of succulent flowering plants. The genera Mammillaria and Notocactus ( Parodia), which have medicinal and nutritional applications as well as aesthetic appeal, are considered to be among the major genera of the family. Several species of both genera show morphological and chemical similarities and diversities according to environmental conditions and genotypes. Here, we assessed the genetic relationships of nine species belonging to two major genera Mammillaria and Notocactus under the family Cactaceae, using two modern gene-targeting marker techniques, the Start Codon Targeted (SCoT) Polymorphism and the Conserved DNA-Derived Polymorphism (CDDP). Besides, we screened the various phytochemicals and evaluated the antioxidant activities of the nine species of cacti. Five out of the 10 SCoT and eight CDDP primers used to screen genetic variations within the nine species yielded species-specific reproducible bands. The entire 156 loci were detected, of which 107 were polymorphic, 26 were monomorphic, and 23 were unique loci. The nine species were categorized into two groups based on the dendrogram and similarity matrix. Phytochemical profiling revealed that sterols, triterpenes, flavonoids, and tannins were found in all the tested species. Additionally, two Notocactus species ( N. shlosserii and N. roseoluteus) and one Mammillaria species ( M. spinosissima) revealed a considerable antioxidant activity. Our results demonstrated that gene-targeting marker techniques were highly powerful tools for the classification and characterization of the nine investigated species, despite displaying high similarities at both morphological and phytochemical levels.

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            Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples

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              Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants


                Author and article information

                Saudi J Biol Sci
                Saudi J Biol Sci
                Saudi Journal of Biological Sciences
                12 March 2020
                June 2020
                12 March 2020
                : 27
                : 6
                : 1649-1658
                [a ]Botany Department, Faculty of Science, Ain-shams University, Egypt
                [b ]Molecular Genetics and Genome Mapping Lab., Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Egypt
                [c ]Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Egypt
                [d ]Pharmacy Department, King Abdullah medical complex, Jeddah, Saudi Arabia
                [e ]Prince Sultan Research Chair for Environment and Wildlife, Department of Botany & Microbiology, College of Sciences, King Saud University (KSU), Riyadh, Saudi Arabia
                [f ]Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia
                [g ]Research Laboratories Center, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia
                [h ]Microbial Genetics Department, Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt
                Author notes
                [* ]Corresponding authors at: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia (G.H. Osman). matia@ 123456ageri.sci.eg geosman@ 123456uqu.edu.sa

                Equal contribution with the 1st author.

                © 2020 The Author(s)

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).


                cactaceae,authentication,genetic diversity,scot,cddp,phytochemical screening


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