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      Draft Genome Sequence of Candida auris Strain LOM, a Human Clinical Isolate from Greater Metropolitan Houston, Texas

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          Abstract

          Candida auris is an emerging pathogen of considerable public health importance. We present the draft genome sequence of a strain recently cultured from the urine of a patient hospitalized in the greater Houston metropolitan region. Two combined Oxford Nanopore sequencing runs provided sufficient data to rapidly generate a draft genome.

          ABSTRACT

          Candida auris is an emerging pathogen of considerable public health importance. We present the draft genome sequence of a strain recently cultured from the urine of a patient hospitalized in the greater Houston metropolitan region. Two combined Oxford Nanopore sequencing runs provided sufficient data to rapidly generate a draft genome.

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          Candida auris: a Review of the Literature

          The emerging pathogen Candida auris has been associated with nosocomial outbreaks on five continents. Genetic analysis indicates the simultaneous emergence of separate clades of this organism in different geographical locations. Invasive infection and colonization have been detected predominantly in patients in high-dependency settings and have garnered attention due to variable antifungal resistance profiles and transmission within units instituting a range of infection prevention and control measures. Issues with the identification of C. auris using both phenotypic and molecular techniques have raised concerns about detecting the true scale of the problem. This review considers the literature available on C. auris and highlights the key unknowns, which will provide direction for further work in this field.
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            Mauve Assembly Metrics

            Summary: High-throughput DNA sequencing technologies have spurred the development of numerous novel methods for genome assembly. With few exceptions, these algorithms are heuristic and require one or more parameters to be manually set by the user. One approach to parameter tuning involves assembling data from an organism with an available high-quality reference genome, and measuring assembly accuracy using some metrics. We developed a system to measure assembly quality under several scoring metrics, and to compare assembly quality across a variety of assemblers, sequence data types, and parameter choices. When used in conjunction with training data such as a high-quality reference genome and sequence reads from the same organism, our program can be used to manually identify an optimal sequencing and assembly strategy for de novo sequencing of related organisms. Availability: GPL source code and a usage tutorial is at http://ngopt.googlecode.com Contact: aarondarling@ucdavis.edu Supplementary information: Supplementary data is available at Bioinformatics online.
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              Whole-Genome Sequencing of a Human Clinical Isolate of emm28 Streptococcus pyogenes Causing Necrotizing Fasciitis Acquired Contemporaneously with Hurricane Harvey

              ABSTRACT We discovered an emm28 Streptococcus pyogenes isolate causing necrotizing fasciitis in a patient exposed to the floodwaters of Hurricane Harvey in the Houston, TX, metropolitan area in August 2017. The Oxford Nanopore MinION instrument provided sufficient genome sequence data within 1 h of beginning sequencing to close the genome.
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                Author and article information

                Contributors
                Role: Editor
                Journal
                Microbiol Resour Announc
                Microbiol Resour Announc
                ga
                mra
                MRA
                Microbiology Resource Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2576-098X
                20 June 2019
                June 2019
                : 8
                : 25
                : e00532-19
                Affiliations
                [a ]Center for Molecular and Translational Human Infectious Diseases Research, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute and Houston Methodist Hospital, Houston, Texas, USA
                [b ]Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA
                Broad Institute
                Author notes
                Address correspondence to S. Wesley Long, swlong@ 123456houstonmethodist.org , or Randall J. Olsen, rjolsen@ 123456houstonmethodist.org .

                Citation Long SW, Olsen RJ, Nguyen HAT, Ojeda Saavedra M, Musser JM. 2019. Draft genome sequence of Candida auris strain LOM, a human clinical isolate from greater metropolitan Houston, Texas. Microbiol Resour Announc 8:e00532-19. https://doi.org/10.1128/MRA.00532-19.

                Author information
                https://orcid.org/0000-0003-3043-5307
                Article
                MRA00532-19
                10.1128/MRA.00532-19
                6588374
                31221653
                a0ef1700-c2e0-46d2-94dc-4c840b765505
                Copyright © 2019 Long et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                : 8 May 2019
                : 23 May 2019
                Page count
                Figures: 1, Tables: 0, Equations: 0, References: 9, Pages: 3, Words: 1329
                Funding
                Funded by: Fondren Foundation;
                Award Recipient :
                Funded by: Simmons Family Foundation, https://doi.org/10.13039/100002336;
                Award Recipient :
                Categories
                Genome Sequences
                Custom metadata
                June 2019

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