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      Fragile X mental retardation protein stimulates ribonucleoprotein assembly of influenza A virus

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          Abstract

          The ribonucleoprotein (RNP) of the influenza A virus is responsible for the transcription and replication of viral RNA in the nucleus. These processes require interplay between host factors and RNP components. Here, we report that the Fragile X mental retardation protein (FMRP) targets influenza virus RNA synthesis machinery and facilitates virus replication both in cell culture and in mice. We demonstrate that FMRP transiently associates with viral RNP and stimulates viral RNP assembly through RNA-mediated interaction with the nucleoprotein. Furthermore, the KH2 domain of FMRP mediates its association with the nucleoprotein. A point mutation (I304N) in the KH2 domain, identified from a Fragile X syndrome patient, disrupts the FMRP-nucleoprotein association and abolishes the ability of FMRP to participate in viral RNP assembly. We conclude that FMRP is a critical host factor used by influenza viruses to facilitate viral RNP assembly. Our observation reveals a mechanism of influenza virus RNA synthesis and provides insights into FMRP functions.

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          Author and article information

          Journal
          Nature Communications
          Nat Comms
          Springer Nature
          2041-1723
          February 10 2014
          February 10 2014
          : 5
          :
          Article
          10.1038/ncomms4259
          24514761
          a123ef10-0f0f-44d1-ac4d-bb938bf1fa3e
          © 2014
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