Objective This study aim to discover the relationship between serum 25-hydroxyvitamin D and tuberculosis patients, and we achieve new ideas for the prevention and treatment of tuberculosis by analyzed its correlation with body immunity cells.
Methods Fifty-four patients diagnosed with tuberculosis who admitted to Suzhou Fifth People’s Hospital from December 2018 to May 2019 were included in the experimental group, and 60 healthy persons undergoing outpatient medical examinations in our hospital at the same period were selected as the control group. 25-hydroxyvitamin D levels were compared in two groups for discovering the relationship between 25-hydroxyvitamin D and tuberculosis. Spearman correlation was used to analyze the correlation of 25-hydroxyvitamin D with these levels of CD3 +, CD4 +, CD8 + and CD4 +/CD8 + ratio as well as other immune cells in patients with tuberculosis.
Results The serum 25-hydroxyvitamin D level of TB patients 9.75 (5.1, 15.8) ng / mL was significantly lower than that of healthy persons 16.8 (6.8, 32.7) ng / mL ( P<0.001). There was no significant difference in 25(OH)D level between sensitive group and MDR-resistant group ( P>0.05).The serum 25-hydroxyvitamin D level was significantly positively correlated with peripheral blood CD4 + level and CD4 +/CD8 + ratio ( r=0.317 2 and 0.404 4, both P< 0.05), and was significantly negatively correlated to the level of CD8 + cells ( r=- 0.329 9, P=0.015); and positively correlated with NK cell levels ( r=0.326 2, P=0.016), and negatively correlated with B lymphocytes and monocytes ( r=-0.333 9 and -0.301 2, both P<0.05).
Conclusion The serum 25-hydroxyvitamin D is significantly reduced in patients with tuberculosis, which may be involved in the regulation of immune function.
摘要：目的 探究结核病患者外周血 25-羟基维生素 D 的表达水平, 分析其与免疫细胞亚群之间的相关性, 为结核 病防治提供新的思路。 方法 纳入 2018 年 12 月—2019 年 5 月入住苏州市第五人民医院诊断为结核患者 54 例为实验 组, 并随机选取同期进行健康体检者 60 例作为对照组; 通过比较两组人群 25-羟基维生素 D 水平。采用 Spearman 相关 性分析研究结核病患者 25-羟基维生素 D 水平与 CD3 +、CD4 +、CD8 +T 淋巴细胞, CD4 +/CD8 +比例及其他免疫细胞水平的 相关性。 结果 结核病患者血清 25-羟基维生素 D 水平中位数为 9.75 (5.1, 15.8) ng/mL, 较健康对照组人群 16.8 (6.8, 32.7) ng/mL 明显降低( P<0.001), 结核病的敏感组和耐多药组 25(OH)D 水平差异无统计学意义 ( P>0.05) , 且血清 25-羟基 维生素 D 水平与外周血 CD4 +、CD4 +/CD8 +呈明显正相关 ( r=0.317 2、0.404 4, 均 P<0.05) , 与外周血 CD8 +呈明显负相关 ( r=-0.329 9, P=0.015) ; 与 NK 细胞水平呈正相关 ( r=0.326 2, P=0.016), 与 B 淋巴细胞及单核细胞呈负相关 ( r=-0.333 9、-0.301 2, 均 P<0.05)。 结论 结核患者中血清 25-羟基维生素 D 明显降低, 可能参与了机体免疫功能调节。