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      Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste

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          Abstract

          Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 degrees C and retained its activity up to 3days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste. Copyright 2010 Elsevier Ltd. All rights reserved.

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          Author and article information

          Journal
          Bioresource Technology
          Bioresource Technology
          Elsevier BV
          09608524
          June 2010
          June 2010
          : 101
          : 11
          : 4096-4103
          Article
          10.1016/j.biortech.2010.01.036
          20133129
          a13f8711-975a-4438-9f70-43b3e92dd689
          © 2010

          https://www.elsevier.com/tdm/userlicense/1.0/

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