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      The novel loop-mediated isothermal amplification based confirmation methodology on the bacteria in Viable but Non-Culturable (VBNC) state.

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          Abstract

          As a self-protection mechanism, the viable but non-culturable (VBNC) state provides the ability against conventional detection methods among various foodborne pathogens. The ability of forming colonies is lost while metabolism is still maintaining in VBNC state cells. Recently, ethidium monoazide (EMA) and propidium monoazide (PMA) have been widely applied on the detection of foodborne pathogens in VBNC state. Combined with loop-mediated isothermal amplification (LAMP), the PMA/EMA-LAMP showed a significant priority in high sensitivity, specificity and rapidity over conventional PCR based assays. Particularly, PMA/EMA-LAMP has been proved as an effective method in the detection of Escherichia coli, Vibrio parahaemolyticus and Staphylococcus in VBNC state. Based on the current investigations, the VBNC mechanism and current detection method for VBNC-state foodborne pathogens were introduced and discussed in this review.

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          Author and article information

          Journal
          Microb. Pathog.
          Microbial pathogenesis
          Elsevier BV
          1096-1208
          0882-4010
          Oct 2017
          : 111
          Affiliations
          [1 ] Department of Haematology, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou 510623, PR China.
          [2 ] Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, PR China.
          [3 ] Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, PR China. Electronic address: jyksys@126.com.
          [4 ] Department of Haematology, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou 510623, PR China. Electronic address: zhangligz06@163.com.
          Article
          S0882-4010(17)30999-3
          10.1016/j.micpath.2017.09.007
          28888887
          a17bd27f-4815-4684-8769-f0adf81fed37

          VBNC,Foodborne pathogens,LAMP
          VBNC, Foodborne pathogens, LAMP

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