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      Phospholipid Analysis of Mammalian Optic Nerve Tissue: A 31P Nuclear Magnetic Resonance Spectroscopic Study

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          Abstract

          Phospholipids of optic nerve (n = 30) from 5.6-kg rabbits were analyzed by <sup>31</sup>P nuclear magnetic resonance (NMR) spectroscopy. Phospholipid metabolites detected were as follows (mol %): phosphatidylcholine (PC; 25.82 ± 0.12), PCplasmalogen/ alkylacyl PC (2.07 ± 0.13), sphingosylphosphorylcholme (1.12 ± 0.20), phosphatidylinositol (PI; 2.17 ± 0.21), lysoPC (0.85 ± 0.06), sphingomyelin (12.52 ± 0.10); phosphatidyl-serine(PS; 14.38 ± 0.11), phosphatidylethanolamine(8.98 ± 0.11), ethanolamine plasmalogen (28.99 ± 0.30), unidentified phospholipid (1.10 ± 0.01), phosphatidic acid (PA; 1.72 ± 0.06), and lyso PS (0.28 ± 0.10). The bulk of the ethanolamine phosphatide is in the form of its plasmalogen, which is the major phospholipid detected. The choline plasmalogen, or a reduced derivative thereof, also is present; thus, a significant phospholipid biosynthetic pathway for optic nerve tissue involves the plasmalogen route, which is a pathway distinct from the PA route responsible for the synthesis of PS, PI, and PC. This new <sup>31</sup>P NMR lipid analytical technique offers potential for studying optic nerve phospholipid metabolism and degenerating optic nerve tissue, since the technique can accurately quantitate (1) both plasmalogen and nonplasmalogen phospholipids, (2) minor phospholipid components, and (3) previously undetected phospholipids.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1994
          1994
          11 December 2009
          : 26
          : 5
          : 264-274
          Affiliations
          aSchepens Eye Research Institute and bDepartment of Ophthalmology, Harvard Medical School, Boston, Mass., and cMagnetic Resonance Laboratory, Midwestern University, Chicago, Ill., USA
          Article
          267488 Ophthalmic Res 1994;26:264–274
          10.1159/000267488
          7877796
          © 1994 S. Karger AG, Basel

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          Page count
          Pages: 11
          Categories
          Original Paper

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