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      The complete mitochondrial genome of Mekong tiger fish Datnioides undecimradiatus (Roberts & Kottelat, 1994)

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          Abstract

          In this study, the complete mitogenome sequence of the Mekong tiger fish Datnioides undecimradiatus (Perciformes: Datnioididae) has been sequenced by next-generation sequencing method. The assembled mitogenome consisting of 16,534 bp, includes 13 protein-coding genes, 22 transfer RNAs and 2 ribosomal RNAs genes. The overall base composition of the Mekong tiger fish is 27.9% for A, 30.69% for C, 16.51% for G, 24.91% for T and show 77% identities to Anoplocapros lenticularis(GenBank accession no. NC011319.1). The complete mitogenome of the Mekong tiger fish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for Datnioididae family.

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          Most cited references8

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          MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

          We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference by providing a graphical user interface (GUI) to specify the phylogeny and calibration constraints step-by-step. This version also contains enhanced algorithms to search for the optimal trees under evolutionary criteria and implements a more advanced memory management that can double the size of sequence data sets to which MEGA can be applied. Both GUI and command-line versions of MEGA6 can be downloaded from www.megasoftware.net free of charge.
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            Automatic annotation of organellar genomes with DOGMA.

            The Dual Organellar GenoMe Annotator (DOGMA) automates the annotation of organellar (plant chloroplast and animal mitochondrial) genomes. It is a Web-based package that allows the use of BLAST searches against a custom database, and conservation of basepairing in the secondary structure of animal mitochondrial tRNAs to identify and annotate genes. DOGMA provides a graphical user interface for viewing and editing annotations. Annotations are stored on our password-protected server to enable repeated sessions of working on the same genome. Finished annotations can be extracted for direct submission to GenBank.
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              ARWEN: a program to detect tRNA genes in metazoan mitochondrial nucleotide sequences.

              Mitochondrial genomes encode their own transfer RNAs (tRNAs). These are often degenerate in sequence and structure compared to tRNAs in their bacterial ancestors. This is one of the reasons why current tRNA gene predictor programs perform poorly identifying mitochondrial tRNA genes. As a consequence there is a need for a new program with the specific aim of predicting these tRNAs. In this study, we present the software ARWEN that identifies tRNA genes in metazoan mitochondrial nucleotide sequences. ARWEN detects close to 100% of previously annotated genes. An online version, software for download and test results are available at www.acgt.se/online.html
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                Author and article information

                Journal
                Mitochondrial DNA B Resour
                Mitochondrial DNA B Resour
                Mitochondrial DNA. Part B, Resources
                Taylor & Francis
                2380-2359
                5 July 2016
                2016
                : 1
                : 1
                : 367-368
                Affiliations
                [a ]Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture , Shanghai, PR China;
                [b ]Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206), Shanghai Ocean University , Shanghai, PR China
                Author notes
                CONTACT Zaizhong Chen chenzz@ 123456shou.edu.cn Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206), Shanghai Ocean University , 999 Huchenghuan Road, 201306Shanghai, PR China
                Article
                1168720
                10.1080/23802359.2016.1168720
                7799610
                a1b69833-4864-4192-b3b5-c6b5ca78e2c1
                © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Page count
                Figures: 1, Pages: 2
                Categories
                Research Article
                Mitogenome Announcement

                mekong tiger fish,mitogenome,next-generation sequencing

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