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Carcinoembryonic antigen detection with "Handing"-controlled fluorescence spectroscopy using a color matrix for point-of-care applications.

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      Abstract

      In this study, we developed a power-free, accurate, and portable diagnostic platform called Handing based on embedded technology, which can rapidly detect fluorescent signals from quantum dots (QDs) on lateral flow test strips (LFTSs). The Handing system has three components: a hand-held test terminal, LFTS cartridge, and data server. The hand-held test terminal is the primary system component and it is integrated with tiny components using printed circuit board packaging for image acquisition, processing, and data handling by a specific program. A black smooth shell and three-dimensional printed test cartridge are used to facilitate the portability of the detection terminal, which provides a closed detection process as well as enhancing the anti-interference capacity. The functions of the data server comprise pre-editing, storage, range querying, and sharing with an international network. Multiple hand-held terminal devices can be linked simultaneously to the same data server. In this study, we detected the tumor marker carcinoembryonic antigen (CEA) using the QD-LFTS system, which allowed quantitative analysis in the range of 1-100ng/mL with an ideal detection limit of 0.049ng/mL. Thus, the system is suitable for detecting CEA in the clinically accepted range. We also detected 70 positive and 30 negative serum samples using the Handing system, which exhibited good specificity and sensitivity. Thus, Handing has the capacity for rapid quantitative detection with high stability and repeatability, so it could be used for in vitro diagnostics in the laboratory and in other conditions for various applications, e.g., food evaluation, disease screening, environmental monitoring, and drug testing.

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      Affiliations
      [1 ] Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
      [2 ] Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, China. Electronic address: wk_xa@163.com.
      [3 ] Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, Guangdong 510280, China.
      [4 ] School of Naval Architecture, Ocean & Civil Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
      [5 ] Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
      [6 ] Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Shanghai 200240, China. Electronic address: dxcui@sjtu.edu.cn.
      Journal
      Biosens Bioelectron
      Biosensors & bioelectronics
      Elsevier BV
      1873-4235
      0956-5663
      Apr 15 2017
      : 90
      27825889
      S0956-5663(16)31071-5
      10.1016/j.bios.2016.10.052

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