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      Proteome dynamics: revisiting turnover with a global perspective.

      Molecular & Cellular Proteomics : MCP
      Animals, Humans, Isotope Labeling, Proteins, analysis, metabolism, Proteolysis, Proteome, Proteomics, methods, Staining and Labeling

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          Abstract

          Although bulk protein turnover has been measured with the use of stable isotope labeled tracers for over half a century, it is only recently that the same approach has become applicable to the level of the proteome, permitting analysis of the turnover of many proteins instead of single proteins or an aggregated protein pool. The optimal experimental design for turnover studies is dependent on the nature of the biological system under study, which dictates the choice of precursor label, protein pool sampling strategy, and treatment of data. In this review we discuss different approaches and, in particular, explore how complexity in experimental design and data processing increases as we shift from unicellular to multicellular systems, in particular animals.

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          Author and article information

          Journal
          23125033
          3518130
          10.1074/mcp.O112.022186

          Chemistry
          Animals,Humans,Isotope Labeling,Proteins,analysis,metabolism,Proteolysis,Proteome,Proteomics,methods,Staining and Labeling

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