<p class="first" id="d3234294e156">The main objective of this study was to identify
analytes that could change and that
could help to clarify the metabolic and physiopathological changes related to canine
obesity-related metabolic dysfunction (ORMD). For this, serum from 35 overweight/obese
dogs, with and without ORMD, was submitted to a comprehensive panel of biochemistry
analysis, a gel-free tandem mass tag isobaric label-based proteomic analysis, and,
finally, selected proteins were used as a starting point for creating a protein interaction
network. Dogs with ORMD showed significantly higher serum concentrations of alanine
aminotransferase (ALT), alkaline phosphatase (ALP), Ca, total proteins, albumin, total
cholesterol, triglycerides, glucose, and butyrylcholinesterase (BChE) activity in
comparison with dogs without ORMD. Proteomic analysis revealed that 23 proteins related
to lipid metabolism, the complement factor system, cellular adhesion and functionality,
inflammation, and coagulation were altered in dogs with ORMD. Finally, the obtained
protein interaction network highlighted that the central term of this network was
the negative regulation of the immune response. These data suggest that canine ORMD
is associated with changes in analytes that reflect altered lipid metabolism, and
liver and immune function impairment and suggests the potential for a prothrombotic
state and lung function alterations.
</p>