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      Prostaglandins and the Release of LHRH from Hypothalamic Synaptosomes

      , ,

      Neuroendocrinology

      S. Karger AG

      Indomethacin, LHRH, Prostaglandins, Synaptosomes

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          Abstract

          The possible role of prostaglandins (PGs) in the hypothalamic control of ovulation has been investigated by studying the effects of indomethacin (ID) and PGs on the release of luteinizing hormone-releasing hormone (LHRH) from hypothalamic synaptosomes. Female rats were chronically pretreated with ID on diestrus 2 (D<sub>2</sub>) and proestrus (P), or on diestrus 1 (D<sub>1</sub>) and D<sub>2</sub>. Basal LHRH release from hypothalamic synaptosomes prepared from rats pretreated on D<sub>2</sub>/P was reduced to 48% after a 20-min incubation period, compared with sham-treated animals. ID pretreatment on D<sub>1</sub>/D<sub>2</sub> caused a similar but less marked reduction in LHRH release (to 66% of controls at 20 min). Extraction of the LHRH remaining within the synaptosomal pellet showed that ID pretreatment also reduced the synaptosome content of LHRH. (15S)-15-methyl PGE<sub>2</sub> (15-E<sub>2</sub>) (10<sup>-4</sup> and 10<sup>–6</sup> M) added to the incubating medium stimulated the release of LHRH by approximately 40%, but this effect was only observed in synaptosomes prepared from ID-treated rats. PGE<sub>2</sub> (10<sup>–4</sup> and 10<sup>–6</sup> M) had no significant effect in either sham-treated or ID-treated groups.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1979
          1979
          26 March 2008
          : 28
          : 6
          : 394-401
          Affiliations
          Department of Physiology, St. George’s Hospital Medical School, London
          Article
          122888 Neuroendocrinology 1979;28:394–401
          10.1159/000122888
          379680
          © 1979 S. Karger AG, Basel

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          Pages: 8
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