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      A genome-scale shRNA resource for transgenic RNAi in Drosophila.

      Nature Methods

      genetics, RNA, Small Interfering, RNA Interference, Oogenesis, MicroRNAs, Genome, Insect, Genetic Vectors, Genetic Techniques, Gene Knockdown Techniques, Female, Drosophila melanogaster, DNA Primers, Base Sequence, Animals, Genetically Modified, Animals

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          Abstract

          Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

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          Author and article information

          Journal
          21460824
          3489273
          10.1038/nmeth.1592

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