Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS ONE
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
15
April
2019
Publication date Collection: 2019
Volume: 14
Issue: 4
Electronic Location Identifier: e0209881
Affiliations
[1
]
University College Dublin, Dublin, Ireland
[2
]
Mater Misericordiae University Hospital, Dublin, Ireland
[3
]
Vis a Vis Symposiums, Highcliffe, Dorset, United Kingdom
[4
]
University College Cork, Cork, Ireland
[5
]
Milford Molecular Diagnostics, Connecticut, United States of America
Cornell University, UNITED STATES
Author notes
Competing Interests: SHL is Director of Milford Molecular Diagnostics Laboratory. There are no patents,
products in development or marketed products associated with this research to declare.
This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Author information
Article
Publisher ID:
PONE-D-18-34731
DOI: 10.1371/journal.pone.0209881
PMC ID: 6464168
PubMed ID: 30986208
SO-VID: a3104161-f626-4c89-98a0-8bf7389da1a9
Copyright © © 2019 Lambert et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
Milford Molecular Diagnostics Laboratory provided support in the form of salary for
SHL. Milford Molecular Diagnostics is a CLIA-certified laboratory developing DNA sequencing-based
diagnostic tests implementable in community hospital laboratories and providing commercial
tests for Borrelia detection by core genome Sanger Sequencing. SHL contributed the
laboratory investigations to this project. The specific roles of these authors are
articulated in the ‘author contributions’ section. The funder had no additional role
in study design, data collection and analysis, decision to publish, or preparation
of the manuscript.
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Artificial Gene Amplification and Extension
Subject:
Polymerase Chain Reaction
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Artificial Gene Amplification and Extension
Subject:
Polymerase Chain Reaction
Subject:
Medicine and Health Sciences
Subject:
Infectious Diseases
Subject:
Bacterial Diseases
Subject:
Borrelia Infection
Subject:
Biology and Life Sciences
Subject:
Organisms
Subject:
Bacteria
Subject:
Borrelia
Subject:
Borrelia Burgdorferi
Subject:
Biology and Life Sciences
Subject:
Microbiology
Subject:
Medical Microbiology
Subject:
Microbial Pathogens
Subject:
Bacterial Pathogens
Subject:
Borrelia
Subject:
Borrelia Burgdorferi
Subject:
Medicine and Health Sciences
Subject:
Pathology and Laboratory Medicine
Subject:
Pathogens
Subject:
Microbial Pathogens
Subject:
Bacterial Pathogens
Subject:
Borrelia
Subject:
Borrelia Burgdorferi
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
RNA
Subject:
Non-coding RNA
Subject:
Ribosomal RNA
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Ribosomes
Subject:
Ribosomal RNA
Subject:
Biology and life sciences
Subject:
Cell biology
Subject:
Cellular structures and organelles
Subject:
Ribosomes
Subject:
Ribosomal RNA
Subject:
Biology and Life Sciences
Subject:
Organisms
Subject:
Bacteria
Subject:
Borrelia
Subject:
Biology and Life Sciences
Subject:
Microbiology
Subject:
Medical Microbiology
Subject:
Microbial Pathogens
Subject:
Bacterial Pathogens
Subject:
Borrelia
Subject:
Medicine and Health Sciences
Subject:
Pathology and Laboratory Medicine
Subject:
Pathogens
Subject:
Microbial Pathogens
Subject:
Bacterial Pathogens
Subject:
Borrelia
Subject:
Research and analysis methods
Subject:
Extraction techniques
Subject:
DNA extraction
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Artificial Gene Amplification and Extension
Subject:
Polymerase Chain Reaction
Subject:
Nested Polymerase Chain Reaction
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Artificial Gene Amplification and Extension
Subject:
Polymerase Chain Reaction
Subject:
Nested Polymerase Chain Reaction
Subject:
Biology and life sciences
Subject:
Molecular biology
Subject:
Molecular biology techniques
Subject:
Sequencing techniques
Subject:
DNA sequencing
Subject:
Dideoxy DNA sequencing
Subject:
Research and analysis methods
Subject:
Molecular biology techniques
Subject:
Sequencing techniques
Subject:
DNA sequencing
Subject:
Dideoxy DNA sequencing