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      Phylogeny and taxonomy of three new Ctenomyces (Arthrodermataceae, Onygenales) species from China

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          Abstract

          Twelve Ctenomyces ( Arthrodermataceae , Onygenales ) strains were obtained and identified during a survey of keratinophilic fungi in soils from China. We used molecular identification combined with morphological evidence to delimit species, circumscribing five species in the genus. Three new species are herein described: C. albus sp. nov., C. obovatus sp. nov. and C. peltricolor sp. nov. We also described, illustrated and compared the novel species with related species in the morphology.

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          MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability

          We report a major update of the MAFFT multiple sequence alignment program. This version has several new features, including options for adding unaligned sequences into an existing alignment, adjustment of direction in nucleotide alignment, constrained alignment and parallel processing, which were implemented after the previous major update. This report shows actual examples to explain how these features work, alone and in combination. Some examples incorrectly aligned by MAFFT are also shown to clarify its limitations. We discuss how to avoid misalignments, and our ongoing efforts to overcome such limitations.
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            MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

            We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference by providing a graphical user interface (GUI) to specify the phylogeny and calibration constraints step-by-step. This version also contains enhanced algorithms to search for the optimal trees under evolutionary criteria and implements a more advanced memory management that can double the size of sequence data sets to which MEGA can be applied. Both GUI and command-line versions of MEGA6 can be downloaded from www.megasoftware.net free of charge.
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              AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS

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                Author and article information

                Contributors
                Journal
                MycoKeys
                MycoKeys
                MycoKeys
                MycoKeys
                Pensoft Publishers
                1314-4057
                1314-4049
                2019
                20 February 2019
                : 47
                : 1-16
                Affiliations
                [1 ] Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China Guizhou University Guiyang China
                [2 ] Department of Microbiology, Guiyang College of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China Guiyang College of Traditional Chinese Medicine Guiyang China
                Author notes
                Corresponding author: Yan-Feng Han ( swallow1128@ 123456126.com )

                Academic editor: Kevin Hyde

                Author information
                https://orcid.org/0000-0003-2031-7518
                Article
                10.3897/mycokeys.47.30740
                6393398
                30820164
                a32beb88-fe24-49ef-8fb3-4035bf312a72
                Zhi-Yuan Zhang, Yan-Feng Han, Wan-Hao Chen, Zong-Qi Liang

                This is an open access article distributed under the terms of the CC0 Public Domain Dedication.

                History
                : 22 October 2018
                : 29 January 2019
                Categories
                Research Article
                Ascomycota
                Taxonomy
                Asia

                3 new species,filamentous fungi, ctenomyces ,morphology,multigene

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