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      Description of a new horned toad of Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from southwest China

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          Abstract

          A new species of the genus Megophrys is described from Guizhou Province, China. Molecular phylogenetic analyses supported the new species as an independent clade nested into the Megophrys . The new species could be distinguished from its congeners by a combination of the following characters: body size moderate (SVL 49.3–58.2 mm in males); vomerine ridges present distinctly, vomerine teeth present; tongue feebly notched behind; tympanum distinctly visible, oval; two metacarpal tubercles in hand; toes with one-third webbing and wide lateral fringes; heels overlapped when thighs are positioned at right angles to the body; tibiotarsal articulation reaching the level between tympanum and eye when leg stretched forward; an internal single subgular vocal sac present in male; in breeding male, the nuptial pads with large and sparse black nuptial spines present on the dorsal bases of the first two fingers.

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          MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

          We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference by providing a graphical user interface (GUI) to specify the phylogeny and calibration constraints step-by-step. This version also contains enhanced algorithms to search for the optimal trees under evolutionary criteria and implements a more advanced memory management that can double the size of sequence data sets to which MEGA can be applied. Both GUI and command-line versions of MEGA6 can be downloaded from www.megasoftware.net free of charge.
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            New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0.

            PhyML is a phylogeny software based on the maximum-likelihood principle. Early PhyML versions used a fast algorithm performing nearest neighbor interchanges to improve a reasonable starting tree topology. Since the original publication (Guindon S., Gascuel O. 2003. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52:696-704), PhyML has been widely used (>2500 citations in ISI Web of Science) because of its simplicity and a fair compromise between accuracy and speed. In the meantime, research around PhyML has continued, and this article describes the new algorithms and methods implemented in the program. First, we introduce a new algorithm to search the tree space with user-defined intensity using subtree pruning and regrafting topological moves. The parsimony criterion is used here to filter out the least promising topology modifications with respect to the likelihood function. The analysis of a large collection of real nucleotide and amino acid data sets of various sizes demonstrates the good performance of this method. Second, we describe a new test to assess the support of the data for internal branches of a phylogeny. This approach extends the recently proposed approximate likelihood-ratio test and relies on a nonparametric, Shimodaira-Hasegawa-like procedure. A detailed analysis of real alignments sheds light on the links between this new approach and the more classical nonparametric bootstrap method. Overall, our tests show that the last version (3.0) of PhyML is fast, accurate, stable, and ready to use. A Web server and binary files are available from http://www.atgc-montpellier.fr/phyml/.
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              Biodiversity hotspots for conservation priorities.

              Conservationists are far from able to assist all species under threat, if only for lack of funding. This places a premium on priorities: how can we support the most species at the least cost? One way is to identify 'biodiversity hotspots' where exceptional concentrations of endemic species are undergoing exceptional loss of habitat. As many as 44% of all species of vascular plants and 35% of all species in four vertebrate groups are confined to 25 hotspots comprising only 1.4% of the land surface of the Earth. This opens the way for a 'silver bullet' strategy on the part of conservation planners, focusing on these hotspots in proportion to their share of the world's species at risk.
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                Author and article information

                Contributors
                Journal
                Zookeys
                Zookeys
                2
                urn:lsid:arphahub.com:pub:45048D35-BB1D-5CE8-9668-537E44BD4C7E
                urn:lsid:zoobank.org:pub:91BD42D4-90F1-4B45-9350-EEF175B1727A
                ZooKeys
                Pensoft Publishers
                1313-2989
                1313-2970
                2020
                07 October 2020
                : 974
                : 131-159
                Affiliations
                [1 ] College of Forestry, Guizhou University, Guiyang 550002, China Guizhou University Guiyang China
                [2 ] Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China Chinese Academy of Sciences Chengdu China
                [3 ] Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment of China, Nanjing 210042, China Nanjing Institute of Environmental Sciences Nanjing China
                [4 ] Kuankuoshui National Nature Reserve Administration, Suiyang 563300, China Kuankuoshui National Nature Reserve Administration Suiyang China
                Author notes
                Corresponding author: Shize Li ( 976722439@ 123456qq.com ); Bin Wang ( wangbin@ 123456cib.ac.cn )

                Academic editor: Anthony Herrel

                Author information
                https://orcid.org/0000-0001-6036-5579
                Article
                56070
                10.3897/zookeys.974.56070
                7557532
                33110381
                a37ee8f6-c37c-4d4d-aa4b-7731624eeb4c
                Haijun Su, Shengchao Shi, Yanqing Wu, Guangrong Li, Xiaogang Yao, Bin Wang, Shize Li

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 01 July 2020
                : 24 August 2020
                Funding
                This work was supported by National Natural Sciences Foundation of China (NSFC31960099), Basic research project of science and technology department of Guizhou Province (Nos. [2020] 1Y083), Science and technology support project of science and technology department of Guizhou Provincial (No. [2020] 4Y029) and Guizhou Provincial Department of Education Youth Science and Technology Talents Growth Project (Nos. KY[2018]455 and KY[2018]468).
                Categories
                Research Article
                Amphibia
                Animalia
                Anura
                Vertebrata
                Taxonomy
                Cenozoic
                Neogene
                Asia
                China Seas
                Far East

                Animal science & Zoology
                china,molecular phylogenetic analysis,morphology,new species,taxonomy,animalia,anura,megophrys

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