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      Knockdown of STIM1 inhibits 6-hydroxydopamine-induced oxidative stress through attenuating calcium-dependent ER stress and mitochondrial dysfunction in undifferentiated PC12 cells.

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          Abstract

          Stromal interaction molecule (STIM) proteins are parts of elaborate eukaryotic Ca(2+) signaling systems and are considered to be important players in regulating neuronal Ca(2+) homeostasis under normal ageing and pathological conditions. Here, we investigated the potential role of STIM1 in 6-hydroxydopamine (6-OHDA)-induced toxicity in undifferentiated PC12 cell lines. Cells exposed to 6-OHDA demonstrated alterations in the generation of reactive oxygen species (ROS) in a Ca(2+)-dependent manner. Downregulation of STIM1 expression by specific small interfering RNA (siRNA) attenuated apoptotic cell death, reduced intracellular ROS production, and partially prevented the impaired endogenous antioxidant enzyme activities after 6-OHDA treatment. Furthermore, STIM1 knockdown significantly attenuated 6-OHDA-induced intracellular Ca(2+) overload by inhibiting endogenous store-operated calcium entry (SOCE). The effect of STIM1 siNRA on SOCE was related to orai1 and L-type Ca(2+) channels, but not to transient receptor potential canonical type 1 (TRPC1) channel. In addition, silencing of STIM1 increased the Ca(2+) buffering capacity of the endoplasmic reticulum (ER) in 6-OHDA-injured cells. ER vacuoles formed from the destruction of ER structural integrity and activation of ER-related apoptotic factors (CHOP and Caspase-12) were partially prevented by STIM1 knockdown. Moreover, STIM1 knockdown attenuated 6-OHDA-induced mitochondrial Ca(2+) uptake and mitochondrial dysfunction, including the collapse of mitochondrial membrane potential (MMP) and the decrease of ATP generation. Taken together, our data provide the first evidence that inhibition of STIM1-meditated intracellular Ca(2+) dyshomeostasis protects undifferentiated PC12 cells against 6-OHDA toxicity and indicate that STIM1 may be responsible for neuronal oxidative stress induced by ER stress and mitochondrial dysfunction in PD.

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          Author and article information

          Journal
          Free Radic. Res.
          Free radical research
          Informa UK Limited
          1029-2470
          1029-2470
          Jul 2014
          : 48
          : 7
          Affiliations
          [1 ] Department of Reproductive Medicine, the First Affiliated Hospital of Medical School, Xi'an Jiaotong University , Xi'an, Shaanxi , The People's Republic of China.
          Article
          10.3109/10715762.2014.905687
          24720513
          a3927134-37e3-4823-af2c-263cc2852671
          History

          ER stress,STIM1,mitochondrial dysfunction,reactive oxygen species,store-operated calcium entry

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