5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Electrospun poly-l-lactic acid/polyvinyl alcohol nanofibers improved insulin-producing cell differentiation potential of human adipose-derived mesenchymal stem cells.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Combination of adipose-derived mesenchymal stem cells (ADSCs) and synthetic materials in terms of pancreatic tissue engineering can be considered as a treatment of diabetes. This study aimed to evaluate the differentiation of human ADSCs to pancreatic cells on poly-l-lactic acid/polyvinyl alcohol (PLLA/PVA) nanofibers as a three-dimensional (3D) scaffold. Mesenchymal stem cells (MSCs) were characterized for mesenchymal surface markers by flow cytometry. Then ADSCs were seeded on 3D scaffolds and treated with pancreatic differentiation medium. Immunostaining assay showed that ADSCs were very efficiently differentiated into a relatively homogeneous population of insulin-producing cells. Moreover, real-time RT-PCR results revealed that pancreas-specific markers were highly expressed in 3D scaffolds compared with their expression in tissue culture plates and this difference in expression level was significant. In addition, insulin and C-peptide secreted in response to varying concentrations of glucose in the 3D scaffold group was significantly higher than that in 2D culture. The results of the present study confirmed that PLLA/PVA scaffold seeded with ADSCs could be a suitable option in pancreatic tissue engineering.

          Related collections

          Author and article information

          Journal
          J. Cell. Biochem.
          Journal of cellular biochemistry
          Wiley
          1097-4644
          0730-2312
          June 2019
          : 120
          : 6
          Affiliations
          [1 ] Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
          [2 ] Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
          [3 ] Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
          [4 ] Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.
          [5 ] Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
          [6 ] Stem Cell Technology Research Center, Tehran, Iran.
          Article
          10.1002/jcb.28274
          30548348
          a39da7b2-33f0-40c4-aefe-24330a3574ed
          History

          poly-l-lactic acid/polyvinyl alcohol (PLLA/PVA),insulin-producing cells,differentiation,adipose-derived mesenchymal stem cells (ADSCs),scaffold,pancreatic tissue engineering

          Comments

          Comment on this article