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      A Subset of Neurons Containing Immunoreactive Prolactin Is a Target for Estrogen Regulation of Gene Expression in Rat Hypothalamus

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          Abstract

          Cells whose nuclei accumulated <sup>3</sup>H-estradiol were identified autoradiographically in fixed, frozen sections of colchicine-treated rat hypothalamus (n = 3 animals). After autoradiogramm development, these sections were subjected to immunocytochemistry using rabbit antirat prolactin antiserum and the avidin-biotinylated horseradish peroxidase method. In the hypothalamus, a substantial subset of the neurons containing immunoreactive prolactin accumulated <sup>3</sup>H-estradiol in their nuclei: of 3,642 immunoreactive cells examined, 1,216 had autoradiographically labeled nuclei, or about 33%. The immunoreactive prolactin neurons with autoradiographically labeled nuclei were located in the medial basal hypothalamus intermingled with immunoreactive prolactin neurons whose nuclei were not labeled autoradiographically. Since hypothalamic immunoreactive prolactin neurons have a rich and widely distributed fiber system, the present results suggest that estrogen, acting through a subset of these neurons, can modify directly the neuronal activity of several brain regions which regulate diverse aspects of the reproductive effort. Also, since immonoreactive prolactin and immunoreactive β-endorphin exist in the same hypothalamic cell population, opioid peptides derived from pro-opiomelanocortin may mediate some effects of estrogen on the neural circuitry regulating reproduction.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1989
          1989
          02 April 2008
          : 49
          : 1
          : 23-27
          Affiliations
          Rockefeller University, New York N.Y., USA
          Article
          125086 Neuroendocrinology 1989;49:23–27
          10.1159/000125086
          2716946
          © 1989 S. Karger AG, Basel

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          Page count
          Pages: 5
          Categories
          Original Paper

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