Effect of exposure to CeO 2  nanoparticles on ram spermatozoa during storage at 4 °C for 96 hours

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Cerium oxide nanoparticles (CeO ₂ NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO ₂ NPs for 96 h on the main structural and kinematic parameters of spermatozoa.

Methods

The ejaculates of 5 Sarda rams were collected, pooled and diluted in a soybean lecithin extender. Samples were exposed to increasing doses of CeO ₂ NPs (0, 44 and 220 μg/mL) and stored at 4 °C for 96 h. Analyses of kinematic parameters (computer assisted sperm analysis, CASA), integrity of membranes (PI/PSA staining), ROS production (H ₂DCFDA staining) and DNA damage (sperm chromatin structure assay with acridine orange, SCSA) were performed every 24 h (0, 24, 48, 72 and 96 h of incubation). The experiment was carried out in 6 replicates. Data were analysed by repeated measures ANOVA with Bonferroni’s as post hoc test. When the assumption of normality was not met (ROS), non-parametric Kruskal-Wallis rank test was carried out.

Results

Exposure of ram spermatozoa to increasing doses of CeO ₂ NPs had a beneficial effect on the main motility parameters from 48 h of incubation onward. Velocity of sperm cells was enhanced in the groups exposed to CeO ₂ NPs compared to the control. Incubation with NPs had beneficial effects on the integrity of plasma membranes of spermatozoa, with higher percentage of damaged cells in the control group compared to the exposed ones. Production of ROS was not affected by exposure to NPs and its levels rose at 96 h of incubation. The integrity of DNA remained stable throughout the 96 h of storage regardless of co-incubation with NPs.

Conclusions

We reported beneficial effects of CeO ₂ NPs on kinematic and morphologic parameters of ram semen, such as motility and membrane integrity following 96 h of exposure. Furthermore, we also proved no genotoxic effects of CeO ₂ NPs. These effects could not be related to an antioxidant activity of CeO ₂ NPs, since ROS levels in exposed cells were similar to those of unexposed ones.

Related collections

Most cited references 33

Record: found
Abstract: found
Article: not found

The role of cerium redox state in the SOD mimetic activity of nanoceria.

Eric Heckert, Ajay Karakoti, Sudipta Seal … (2008)

Cerium oxide nanoparticles (nanoceria) have recently been shown to protect cells against oxidative stress in both cell culture and animal models. Nanoceria has been shown to exhibit superoxide dismutase (SOD) activity using a ferricytochrome C assay, and this mimetic activity that has been postulated to be responsible for cellular protection by nanoceria. The nature of nanoceria's antioxidant properties, specifically what physical characteristics make nanoceria effective at scavenging superoxide anion, is poorly understood. In this study electron paramagnetic resonance (EPR) analysis confirms the reactivity of nanoceria as an SOD mimetic. X-ray photoelectron spectroscopy (XPS) and UV-visible analyses of nanoceria treated with hydrogen peroxide demonstrate that a decrease in the Ce 3(+)/4(+) ratio correlates directly with a loss of SOD mimetic activity. These results strongly suggest that the surface oxidation state of nanoceria plays an integral role in the SOD mimetic activity of nanoceria and that ability of nanoceria to scavenge superoxide is directly related to cerium(III) concentrations at the surface of the particle.

0 comments Cited 240 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Hazard and risk assessment of a nanoparticulate cerium oxide-based diesel fuel additive - a case study.

Barry Park, Myrsini Giannouli, Robert Guest … (2008)

Envirox is a scientifically and commercially proven diesel fuel combustion catalyst based on nanoparticulate cerium oxide and has been demonstrated to reduce fuel consumption, greenhouse gas emissions (CO(2)), and particulate emissions when added to diesel at levels of 5 mg/L. Studies have confirmed the adverse effects of particulates on respiratory and cardiac health, and while the use of Envirox contributes to a reduction in the particulate content in the air, it is necessary to demonstrate that the addition of Envirox does not alter the intrinsic toxicity of particles emitted in the exhaust. The purpose of this study was to evaluate the safety in use of Envirox by addressing the classical risk paradigm. Hazard assessment has been addressed by examining a range of in vitro cell and cell-free endpoints to assess the toxicity of cerium oxide nanoparticles as well as particulates emitted from engines using Envirox. Exposure assessment has taken data from modeling studies and from airborne monitoring sites in London and Newcastle adjacent to routes where vehicles using Envirox passed. Data have demonstrated that for the exposure levels measured, the estimated internal dose for a referential human in a chronic exposure situation is much lower than the no-observed-effect level (NOEL) in the in vitro toxicity studies. Exposure to nano-size cerium oxide as a result of the addition of Envirox to diesel fuel at the current levels of exposure in ambient air is therefore unlikely to lead to pulmonary oxidative stress and inflammation, which are the precursors for respiratory and cardiac health problems.

0 comments Cited 67 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells.

Nana Asare, Christine Instanes, Wiggo Sandberg … (2012)

Serious concerns have been expressed about potential risks of engineered nanoparticles. Regulatory health risk assessment of such particles has become mandatory for the safe use of nanomaterials in consumer products and medicines; including the potential effects on reproduction and fertility, are relevant for this risk evaluation. In this study, we examined effects of silver particles of nano- (20nm) and submicron- (200nm) size, and titanium dioxide nanoparticles (TiO(2)-NPs; 21nm), with emphasis on reproductive cellular- and genotoxicity. Ntera2 (NT2, human testicular embryonic carcinoma cell line), and primary testicular cells from C57BL6 mice of wild type (WT) and 8-oxoguanine DNA glycosylase knock-out (KO, mOgg1(-/-)) genotype were exposed to the particles. The latter mimics the repair status of human testicular cells vs oxidative damage and is thus a suitable model for human male reproductive toxicity studies. The results suggest that silver nano- and submicron-particles (AgNPs) are more cytotoxic and cytostatic compared to TiO(2)-NPs, causing apoptosis, necrosis and decreased proliferation in a concentration- and time-dependent manner. The 200nm AgNPs in particular appeared to cause a concentration-dependent increase in DNA-strand breaks in NT2 cells, whereas the latter response did not seem to occur with respect to oxidative purine base damage analysed with any of the particles tested. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

0 comments Cited 65 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Laura Falchi: lfalchi@uniss.it

Grazia Galleri: galleri@uniss.it

Gian Mario Dore: gmdore86@gmail.com

Maria Teresa Zedda: zedda@uniss.it

Salvatore Pau: nuvola@uniss.it

Luisa Bogliolo: luis@uniss.it

Federica Ariu: federica@uniss.it

Alessandra Pinna: a.pinna@imperial.ac.uk

Stefano Nieddu: nieddino@hotmail.it

Plinio Innocenzi: plinio@uniss.it

Sergio Ledda: giodi@uniss.it

Journal

Journal ID (nlm-ta): Reprod Biol Endocrinol

Journal ID (iso-abbrev): Reprod. Biol. Endocrinol

Title: Reproductive Biology and Endocrinology : RB&E

Publisher: BioMed Central (London )

ISSN (Electronic): 1477-7827

Publication date (Electronic): 6 March 2018

Publication date PMC-release: 6 March 2018

Publication date Collection: 2018

Volume: 16

Electronic Location Identifier: 19

Affiliations

[1 ]ISNI 0000 0001 2097 9138, GRID grid.11450.31, Dipartimento di Medicina Veterinaria, , Università degli Studi di Sassari, ; Sassari, Italy

[2 ]ISNI 0000 0001 2097 9138, GRID grid.11450.31, Dipartimento di Medicina Clinica e Sperimentale, , Università degli Studi di Sassari, ; Sassari, Italy

[3 ]ISNI 0000 0001 2113 8111, GRID grid.7445.2, Department of Materials, , Imperial College London, ; South Kensington Campus, London, UK

[4 ]ISNI 0000 0001 2097 9138, GRID grid.11450.31, Dipartimento di Chimica e Farmacia, , Università degli Studi di Sassari, ; Sassari, Italy

Article

Publisher ID: 339

DOI: 10.1186/s12958-018-0339-9

PMC ID: 5840694

PubMed ID: 29510737

SO-VID: a3ca2955-40c2-4c8a-aa0c-efba527b99c6

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 11 December 2017

Date accepted : 2 March 2018

Custom metadata

ScienceOpen disciplines: Human biology

Keywords: acrosome,casa,dna,motility,nanoparticles,ovine,oxidative stress,ros,spermatozoa

Data availability:

ScienceOpen disciplines: Human biology

Keywords: acrosome, casa, dna, motility, nanoparticles, ovine, oxidative stress, ros, spermatozoa

Effect of exposure to CeO ₂ nanoparticles on ram spermatozoa during storage at 4 °C for 96 hours

Read this article at

Abstract

Background

Methods

Results

Conclusions

Related collections

Stress signalling in stem cells

Most cited references 33

The role of cerium redox state in the SOD mimetic activity of nanoceria.

Hazard and risk assessment of a nanoparticulate cerium oxide-based diesel fuel additive - a case study.

Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells.

Author and article information

Contributors

Journal

Affiliations

Article

History

Categories

Custom metadata

Comments

Comment on this article

Similar content 108

Cited by 11

Most referenced authors 459