28
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides- and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.

          Related collections

          Most cited references12

          • Record: found
          • Abstract: found
          • Article: not found

          Screening and surveillance for the early detection of colorectal cancer and adenomatous polyps, 2008: a joint guideline from the American Cancer Society, the US Multi-Society Task Force on Colorectal Cancer, and the American College of Radiology.

          In the United States, colorectal cancer (CRC) is the third most common cancer diagnosed among men and women and the second leading cause of death from cancer. CRC largely can be prevented by the detection and removal of adenomatous polyps, and survival is significantly better when CRC is diagnosed while still localized. In 2006 to 2007, the American Cancer Society, the US Multi-Society Task Force on Colorectal Cancer, and the American College of Radiology came together to develop consensus guidelines for the detection of adenomatous polyps and CRC in asymptomatic average-risk adults. In this update of each organization's guidelines, screening tests are grouped into those that primarily detect cancer early and those that can detect cancer early and also can detect adenomatous polyps, thus providing a greater potential for prevention through polypectomy. When possible, clinicians should make patients aware of the full range of screening options, but at a minimum they should be prepared to offer patients a choice between a screening test that primarily is effective at early cancer detection and a screening test that is effective at both early cancer detection and cancer prevention through the detection and removal of polyps. It is the strong opinion of these 3 organizations that colon cancer prevention should be the primary goal of screening.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Fusobacterium nucleatum associates with stages of colorectal neoplasia development, colorectal cancer and disease outcome.

            Commensal bacteria in the colon may play a role in colorectal cancer (CRC) development. Recent studies from North America showed that Fusobacterium nucleatum (Fn) infection is over-represented in disease tissue versus matched normal tissue in CRC patients. Using quantitative real-time polymerase chain reaction (qPCR) of DNA extracted from colorectal tissue biopsies and surgical resections of three European cohorts totalling 122 CRC patients, we found an over-abundance of Fn in cancerous compared to matched normal tissue (p < 0.0001). To determine whether Fn infection is an early event in CRC development, we assayed Fn in colorectal adenoma (CRA) tissue from 52 Irish patients. While for all CRAs the Fn level was not statistically significantly higher in disease versus normal tissue (p = 0.06), it was significantly higher for high-grade dysplasia (p = 0.015). As a secondary objective, we determined that CRC patients with low Fn levels had a significantly longer overall survival time than patients with moderate and high levels of the bacterium (p = 0.008). The investigation of Fn as a potential non-invasive biomarker for CRC screening showed that, while Fn was more abundant in stool samples from CRC patients compared to adenomas or controls, the levels in stool did not correlate with cancer or adenoma tissue levels from the same individuals. This is the first study examining Fn in the colonic tissue and stool of European CRC and CRA patients, and suggests Fn as a novel risk factor for disease progression from adenoma to cancer, possibly affecting patient survival outcomes. Our results highlight the potential of Fn detection as a diagnostic and prognostic determinant in CRC patients.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Random comparison of guaiac and immunochemical fecal occult blood tests for colorectal cancer in a screening population.

              Despite poor performance, guaiac-based fecal occult blood tests (G-FOBT) are most frequently implemented for colorectal cancer screening. Immunochemical fecal occult blood tests (I-FOBT) are claimed to perform better, without randomized comparison in screening populations. Our aim was to randomly compare G-FOBT with I-FOBT in a screening population. We conducted a population-based study on a random sample of 20,623 individuals 50-75 years of age, randomized to either G-FOBT (Hemoccult-II) or I-FOBT (OC-Sensor). Tests and invitations were sent together. For I-FOBT, the standard cutoff of 100 ng/ml was used. Positive FOBTs were verified with colonoscopy. Advanced adenomas were defined as >or=10 mm, high-grade dysplasia, or >or=20% villous component. There were 10,993 tests returned: 4836 (46.9%) G-FOBTs and 6157 (59.6%) I-FOBTs. The participation rate difference was 12.7% (P < .01). Of G-FOBTs, 117 (2.4%) were positive versus 339 (5.5%) of I-FOBTs. The positivity rate difference was 3.1% (P < .01). Cancer and advanced adenomas were found, respectively, in 11 and 48 of G-FOBTs and in 24 and 121 of I-FOBTs. Differences in positive predictive value for cancer and advanced adenomas and cancer were, respectively, 2.1% (P = .4) and -3.6% (P = .5). Differences in specificities favor G-FOBT and were, respectively, 2.3% (P < .01) and -1.3% (P < .01). Differences in intention-to-screen detection rates favor I-FOBT and were, respectively, 0.1% (P < .05) and 0.9% (P < .01). The number-to-scope to find 1 cancer was comparable between the tests. However, participation and detection rates for advanced adenomas and cancer were significantly higher for I-FOBT. G-FOBT significantly underestimates the prevalence of advanced adenomas and cancer in the screening population compared with I-FOBT.
                Bookmark

                Author and article information

                Contributors
                huanghsienda@cuhk.edu.cn
                yjjong@kmu.edu.tw
                forest65@csmu.edu.tw
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                29 July 2019
                29 July 2019
                2019
                : 9
                : 10923
                Affiliations
                [1 ]ISNI 0000 0004 0638 9256, GRID grid.411645.3, Division of Gastroenterology and Hepatology, Department of Internal Medicine, , Chung Shan Medical University Hospital, ; Taichung, 402 Taiwan
                [2 ]ISNI 0000 0004 0532 2041, GRID grid.411641.7, School of Medicine, , Chung Shan Medical University, ; Taichung, 402 Taiwan
                [3 ]ISNI 0000 0001 2059 7017, GRID grid.260539.b, Institute and Department of Biological Science and Technology, College of Biological Science and Technology, , National Chiao Tung University, ; Hsinchu, 300 Taiwan
                [4 ]ISNI 0000 0001 2059 7017, GRID grid.260539.b, Institute of Bioinformatics and Systems Biology, College of Biological Science and Technology, , National Chiao Tung University, ; Hsinchu, 300 Taiwan
                [5 ]ISNI 0000 0004 0638 9256, GRID grid.411645.3, Department of Medical Research, , Chung Shan Medical University Hospital, ; Taichung, 402 Taiwan
                [6 ]ISNI 0000 0004 0532 2041, GRID grid.411641.7, Institute of Medicine, , Chung Shan Medical University, ; Taichung, 402 Taiwan
                [7 ]ISNI 0000 0004 0638 9256, GRID grid.411645.3, Division of Colon and Rectum, Department of Surgery, , Chung Shan Medical University Hospital, ; Taichung, 402 Taiwan
                [8 ]ISNI 0000 0004 0638 9256, GRID grid.411645.3, Division of Endocrinology and Metabolism, Department of Internal Medicine, , Chung Shan Medical University Hospital, ; Taichung, 402 Taiwan
                [9 ]ISNI 0000 0000 9476 5696, GRID grid.412019.f, Graduate Institute of Clinical Medicine, College of Medicine, , Kaohsiung Medical University, ; Kaohsiung, 807 Taiwan
                [10 ]ISNI 0000 0000 9476 5696, GRID grid.412019.f, Departments of Pediatrics and Laboratory Medicine, Kaohsiung Medical University Hospital, , Kaohsiung Medical University, ; Kaohsiung, 807 Taiwan
                [11 ]ISNI 0000 0001 2059 7017, GRID grid.260539.b, Institute of Molecular Medicine and Bioengineering, College of Biological Science and Technology, , National Chiao Tung University, ; Hsinchu, 300 Taiwan
                [12 ]ISNI 0000 0004 1937 0482, GRID grid.10784.3a, Warshel Institute For Computational Biology, , The Chinese University of Hong Kong, Shenzhen, 518172, Longgang District, ; Shenzhen, China
                [13 ]ISNI 0000 0004 1937 0482, GRID grid.10784.3a, School of Life and Health Sciences, , The Chinese University of Hong Kong, Shenzhen, 518172, Longgang District, ; Shenzhen, China
                [14 ]ISNI 0000 0004 1937 0482, GRID grid.10784.3a, School of Sciences and Engineering, The Chinese University of Hong Kong, Shenzhen, 518172, Longgang District, ; Shenzhen, China
                Author information
                http://orcid.org/0000-0002-8222-0503
                http://orcid.org/0000-0001-5714-1718
                http://orcid.org/0000-0002-0365-7927
                http://orcid.org/0000-0003-2857-7023
                Article
                45588
                10.1038/s41598-019-45588-z
                6662695
                31358825
                a3cf172b-09a7-43f2-a3f4-09d1a94de2ca
                © The Author(s) 2019

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 9 May 2018
                : 6 June 2019
                Funding
                Funded by: FundRef https://doi.org/10.13039/501100004663, Ministry of Science and Technology, Taiwan (Ministry of Science and Technology of Taiwan);
                Award ID: MOST 103-2628-B-009-001-MY3
                Award ID: MOST 105-2627-M-009-007
                Award ID: MOST 105-2319-B-400-002
                Award ID: MOST 104-2911-I-009-509
                Award ID: MOST 106-2633-B-009-001
                Award ID: MOST 106-2627-M-009-002
                Award ID: MOST 106-2319-B-400-001
                Award Recipient :
                Funded by: FundRef https://doi.org/10.13039/100008903, Ministry of Health and Welfare (Ministry of Health and Welfare, Taiwan);
                Award ID: MOHW 103-TD-B-111-08
                Award ID: MOHW 104-TDU-B-212-124-005
                Award ID: MOHW 105-TDU-B-212-134002
                Award ID: MOHW 106-TDU-B-212-144005
                Award Recipient :
                Funded by: FundRef https://doi.org/10.13039/501100005799, National Chiao Tung University (NCTU);
                Award ID: NCTU-104W976
                Award ID: NCTU-105W976
                Award Recipient :
                Funded by: FundRef https://doi.org/10.13039/501100004699, CSMU | Chung Shan Medical University Hospital;
                Award ID: CSH-2014-D-004-Y2
                Award Recipient :
                Categories
                Article
                Custom metadata
                © The Author(s) 2019

                Uncategorized
                colon cancer
                Uncategorized
                colon cancer

                Comments

                Comment on this article