For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
24
views
40
references
 Top references
cited by
5
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      154
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Subspecific Differentiation Events of Montane Stag Beetles (Coleoptera, Lucanidae) Endemic to Formosa Island

      research-article
      Author(s): , *
      Editor(s):
      Publication date (Electronic):
      Journal: PLoS ONE
      Publisher: Public Library of Science

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Taxonomic debates have been carrying on for decades over Formosan stag beetles, which consist of a high proportion of endemic species and subspecies featuring morphological variations associated with local adaptation. With the influence of periodical Pleistocene glaciations and the presence of several mountain ranges, the genetic differentiation and taxonomic recognition, within this medium-size island, of two endemic subspecies for each of four montane stag beetles, i.e. Lucanus ogakii, L. kanoi, Prismognathus davidis, and Neolucanus doro, has been an appealing issue. Based on monophyletic lineages and population structure, possible divergent scenarios have been proposed to clarify the subspecific status for each of the above mentioned stag beetles. Phylogenetic inferences based on COI+16S rDNA+28S rDNA of 240 Formosan lucanids have confirmed most species are monophyletic groups; and the intraspecific (<2%) and interspecific (>2%) genetic distances of the two mitochondrial genes could be applied concordantly for taxonomic identification. On account of Bayesian-based species delimitation, geographic distribution, population structure, and sequence divergences, the subspecific status for L. ogakii, L. kanoi, and Pri. davidis are congruent with their geographic distribution in this island; and the calibration time based on the mitochondrial genes shows the subspecific split events occurred 0.7–1 million years ago. In addition, a more complicated scenario, i.e. genetic differentiation including introgression/hybridization events, might have occurred among L. ogakii, L. kanoi, and L. maculifemoratus. The geological effects of mountain hindrance accompanied by periodical glaciations could have been vital in leading to the geographical subspecific differentiation of these montane stag beetles.

          Related collections

          Most cited references40

          • Record: found
          • Abstract: found
          • Article: not found

          Revisiting the insect mitochondrial molecular clock: the mid-Aegean trench calibration.

          Anna Papadopoulou, Ioannis Anastasiou, Alfried Vogler (2010)
          Phylogenetic trees in insects are frequently dated by applying a "standard" mitochondrial DNA (mtDNA) clock estimated at 2.3% My(-1), but despite its wide use reliable calibration points have been lacking. Here, we used a well-established biogeographic barrier, the mid-Aegean trench separating the western and eastern Aegean archipelago, to estimate substitution rates in tenebrionid beetles. Cytochrome oxidase I (cox1) for six codistributed genera across 28 islands (444 individuals) on both sides of the mid-Aegean trench revealed 60 independently coalescing entities delimited with a mixed Yule-coalescent model. One representative per entity was used for phylogenetic analysis of mitochondrial (cox1, 16S rRNA) and nuclear (Mp20, 28S rRNA) genes. Six nodes marked geographically congruent east-west splits whose separation was largely contemporaneous and likely to reflect the formation of the mid-Aegean trench at 9-12 Mya. Based on these "known" dates, a divergence rate of 3.54% My(-1) for the cox1 gene (2.69% when combined with the 16S rRNA gene) was obtained under the preferred partitioning scheme and substitution model selected using Bayes factors. An extensive survey suggests that discrepancies in mtDNA substitution rates in the entomological literature can be attributed to the use of different substitution models, the use of different mitochondrial gene regions, mixing of intraspecific with interspecific data, and not accounting for variance in coalescent times or postseparation gene flow. Different treatments of these factors in the literature confound estimates of mtDNA substitution rates in opposing directions and obscure lineage-specific differences in rates when comparing data from various sources.
          Article 0 comments Cited 305 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae)

            Martin Wiemers, Konrad Fiedler (2007)
            Background DNA barcoding, i.e. the use of a 648 bp section of the mitochondrial gene cytochrome c oxidase I, has recently been promoted as useful for the rapid identification and discovery of species. Its success is dependent either on the strength of the claim that interspecific variation exceeds intraspecific variation by one order of magnitude, thus establishing a "barcoding gap", or on the reciprocal monophyly of species. Results We present an analysis of intra- and interspecific variation in the butterfly family Lycaenidae which includes a well-sampled clade (genus Agrodiaetus) with a peculiar characteristic: most of its members are karyologically differentiated from each other which facilitates the recognition of species as reproductively isolated units even in allopatric populations. The analysis shows that there is an 18% overlap in the range of intra- and interspecific COI sequence divergence due to low interspecific divergence between many closely related species. In a Neighbour-Joining tree profile approach which does not depend on a barcoding gap, but on comprehensive sampling of taxa and the reciprocal monophyly of species, at least 16% of specimens with conspecific sequences in the profile were misidentified. This is due to paraphyly or polyphyly of conspecific DNA sequences probably caused by incomplete lineage sorting. Conclusion Our results indicate that the "barcoding gap" is an artifact of insufficient sampling across taxa. Although DNA barcodes can help to identify and distinguish species, we advocate using them in combination with other data, since otherwise there would be a high probability that sequences are misidentified. Although high differences in DNA sequences can help to identify cryptic species, a high percentage of well-differentiated species has similar or even identical COI sequences and would be overlooked in an isolated DNA barcoding approach.
            Article 0 comments Cited 161 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Neotropical biodiversity: timing and potential drivers.

              Valentí Rull (2011)
              The origin of extant neotropical biodiversity has been a controversial topic since the time of Darwin. In this review, I discuss the timing of, and potential driving factors associated with, diversification using recent evidence from molecular phylogenetics. Although these studies provide new insights into the subject, they are sensitive to dating approaches and targets, and can eventually lead to biased conclusions. A careful analysis suggests that the origin of extant neotropical biodiversity cannot be attributed to the action of one or few events during key time intervals. Rather, it is the result of complex ecological and evolutionary trends initiated by Neogene tectonic events and palaeogeographical reorganisations, and maintained by the action of Pleistocene climatic changes. Copyright © 2011 Elsevier Ltd. All rights reserved.
              Article 0 comments Cited 141 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Tzen-Yuh Chiang: Role: Editor
                Journal
                Journal ID (nlm-ta): PLoS One
                Journal ID (iso-abbrev): PLoS ONE
                Journal ID (publisher-id): plos
                Journal ID (pmc): plosone
                Title: PLoS ONE
                Publisher: Public Library of Science (San Francisco, CA USA )
                ISSN (Electronic): 1932-6203
                Publication date (Electronic): 3 June 2016
                Publication date Collection: 2016
                Volume: 11
                Issue: 6
                Electronic Location Identifier: e0156600
                Affiliations
                [001]Department of Entomology, National Chung Hsing University, Taichung, Taiwan
                National Cheng-Kung University, TAIWAN
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Conceived and designed the experiments: CLT WBY. Performed the experiments: CLT. Analyzed the data: CLT. Contributed reagents/materials/analysis tools: CLT WBY. Wrote the paper: CLT WBY. Specimen collections: CLT.

                Author information
                http://orcid.org/0000-0003-4319-686X
                Article
                Publisher ID: PONE-D-15-51982
                DOI: 10.1371/journal.pone.0156600
                PMC ID: 4892689
                PubMed ID: 27257861
                SO-VID: a4037b5e-4a79-4fde-99b3-b0e048d5e16c
                Copyright © © 2016 Tsai, Yeh
                License:

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 30 November 2015
                Date accepted : 17 May 2016
                Page count
                Figures: 8, Tables: 1, Pages: 23
                Funding
                These authors have no support or funding to report.
                Categories
                Subject: Research Article
                Subject: Biology and Life Sciences
                Subject: Organisms
                Subject: Animals
                Subject: Invertebrates
                Subject: Arthropoda
                Subject: Insects
                Subject: Beetles
                Subject: Biology and Life Sciences
                Subject: Taxonomy
                Subject: Computer and Information Sciences
                Subject: Data Management
                Subject: Taxonomy
                Subject: Biology and Life Sciences
                Subject: Paleontology
                Subject: Paleogenetics
                Subject: Earth Sciences
                Subject: Paleontology
                Subject: Paleogenetics
                Subject: Biology and Life Sciences
                Subject: Evolutionary Biology
                Subject: Evolutionary Processes
                Subject: Speciation
                Subject: Species Delimitation
                Subject: Biology and Life Sciences
                Subject: Molecular Biology
                Subject: Molecular Biology Techniques
                Subject: Molecular Biology Assays and Analysis Techniques
                Subject: Phylogenetic Analysis
                Subject: Research and Analysis Methods
                Subject: Molecular Biology Techniques
                Subject: Molecular Biology Assays and Analysis Techniques
                Subject: Phylogenetic Analysis
                Subject: Biology and Life Sciences
                Subject: Biogeography
                Subject: Phylogeography
                Subject: Ecology and Environmental Sciences
                Subject: Biogeography
                Subject: Phylogeography
                Subject: Earth Sciences
                Subject: Geography
                Subject: Biogeography
                Subject: Phylogeography
                Subject: Biology and Life Sciences
                Subject: Evolutionary Biology
                Subject: Population Genetics
                Subject: Phylogeography
                Subject: Biology and Life Sciences
                Subject: Genetics
                Subject: Population Genetics
                Subject: Phylogeography
                Subject: Biology and Life Sciences
                Subject: Population Biology
                Subject: Population Genetics
                Subject: Phylogeography
                Subject: Earth Sciences
                Subject: Geology
                Subject: Geologic Time
                Subject: Cenozoic Era
                Subject: Quaternary Period
                Subject: Pleistocene Epoch
                Subject: People and Places
                Subject: Geographical Locations
                Subject: Asia
                Subject: Taiwan
                Custom metadata
                Data Availability All sequences were deposited in GenBank under the inclusive following accession numbers (COI: LC074471–LC074690, LC091038–LC091039; 16S rDNA: LC074974–LC075188, LC091040–LC091041; 28S rDNA: LC066683–LC066936, LC126100–LC126101); wingless: LC077663–LC077693, LC126084–LC126099).

                ScienceOpen disciplines: Uncategorized
                Data availability:
                ScienceOpen disciplines: Uncategorized

                Comments

                Comment on this article

                scite_

                Similar content154

                See all similar

                Cited by4

                See all cited by

                Most referenced authors1,160

                See all reference authors