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      Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.

      Journal of Clinical Microbiology

      Animals, DNA Primers, DNA, Protozoan, analysis, isolation & purification, Feces, parasitology, Filtration, methods, Giardia, classification, genetics, growth & development, Giardiasis, Humans, Polymerase Chain Reaction, RNA, Ribosomal, Sensitivity and Specificity

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          Abstract

          Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

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          Journal
          17122010
          1829074
          10.1128/JCM.01823-06

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