25 April 2015
The spike (S) protein of porcine hemagglutinating encephalomyelitis virus (PHEV) may mediate infection by binding to a cellular neural cell adhesion molecule (NCAM). This study aimed to identify the crucial domain of the S1 subunit of the S protein that interacts with NCAM.
Three truncated segments (S 1-291, S 277-794 and S 548-868) of the S gene of PHEV and the NCAM gene were cloned individually into the Escherichia coli expression vectors and yeast two-hybrid expression vectors. The interaction between S 1-291, S 277-794, S 548-868 and NCAM were detected by a GST pull-down experiment and yeast two-hybrid assay.
Three fusion proteins (S 1-291, S 277-794 and S 548-868) were screened for their interactions with NCAM by protein-protein interaction assays. The results of these assays clarified that S 277-794 interacted with NCAM, while S 1-291 and S 548-868 did not.