Primarily in this paper, we have thoroughly investigated the psychological status and job burnout of managers in petroleum enterprises. For this purpose, we have assumed managers in petroleum enterprises as the research objects, whereas the overall sampling method was adopted from January 2019 to June 2020. A total of 240 questionnaires were sent out and 236 valid questionnaires were received with a recovery rate of 98.33%. The research objects' general information, psychological health status, and job burnout level were investigated. Among the 236 management personnel in petroleum enterprises, 212 (89.83%) were male and 24 (10.17%) were female. The average age was (40.28 ± 7.07) years old, and the number of people aged 40–49 was the largest, accounting for 52.12%; 84.32% were in marriage; regular college or above accounted for 81.78%; no smoking or occasional smoking in the last one year was 52.97%; not drinking in the last year was 34.75%; and 60.17% of managers in petroleum enterprises never or occasionally exercise. 121 (51.27%) suffered from high burnout due to emotional exhaustion, 125 (52.97%) suffered from high burnout due to emotional alienation, and 139 (58.90%) suffered from high burnout due to reduced sense of achievement. The scores of somatization, obsessive symptoms, depression, anxiety, and terror of the respondents were significantly higher than the normal levels of domestic adults. The differences were statistically significant (P 0.05). The phenomenon of job burnout and some psychological health problems existed in the managers in petroleum enterprises. The incidence of psychological evaluation was higher than that of the national norm, and the level of psychological health could predict the job burnout of petroleum enterprise managers.