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      Evaluation of polyhydroxyalkanoate (PHAs) production with a bacterial isolate using cassava flour hydrolysates as an alternative substrate Translated title: Evaluación de la producción de polihidroxialcanoatos (PHAs) de un aislado bacteriano, empleando hidrolizados de harina de yuca como sustrato alternativo

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          Abstract

          Abstract In the production of polyhydroxyalkanoates (PHAs), the costs of the process are mainly associated with the carbon source and most production studies are carried out with commercial strains. In this study, PHAs production was evaluated using SB-34, a wild-type strain, in comparison with Ralstonia eutropha H16, a referenced strain growing in cassava flour hydrolysates, as an alternative carbon source. SB-34 reached a maximum biomass of 5,49 ± 0,21 g / L, and a PHAs production of 3,23 ± 0,21 g / L, exceeding the maximum values obtained by R. eutropha H16 (3,8 ± 0,45 g / L and 2,42 ± 0,23 g / L, respectively). Analyses with FTIR indicated that the polymer obtained with SB-34 is a P(3-HB) polyhydroxybutyrate. A molecular analysis identified SB-34 as Burkholderia sp. with 96 % of similarity.

          Translated abstract

          Resumen En la producción de polihidroxialcanoatos (PHAs), los costos del proceso se asocian principalmente con la fuente de carbono y la mayoría de los estudios de producción se realizan con cepas comerciales. En este estudio, se evaluó la producción de PHAs utilizando una cepa silvestre, SB-34, en comparación con una cepa de referencia, Ralstonia eutropha H16, creciendo en hidrolizados de harina de yuca como fuente de carbono alternativa. SB-34 alcanzó un máximo de biomasa de 5.49 ± 0.21 g / L, y un PHAs de 3.23 ± 0.21 g / L que excede los valores máximos obtenidos por R. eutropha H16 (3.8 ± 0.45 g / L y 2.42 ± 0.23 g / L, respectivamente). Los análisis con FTIR indican que el polímero obtenido con SB-34 es del tipo polihidroxibutirato P (3-HB). El análisis molecular identificó al SB-34 como Burkholderia sp. con 96% de similitud.

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          Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.

          Polyhydroxyalkanoates (PHAs), of which polyhydroxybutyrate (PHB) is the most abundant, are bacterial carbon and energy reserve materials of widespread occurrence. They are composed of 3-hydroxyacid monomer units and exist as a small number of cytoplasmic granules per cell. The properties of the C4 homopolymer PHB as a biodegradable thermoplastic first attracted industrial attention more than 20 years ago. Copolymers of C4 (3-hydroxybutyrate [3HB]) and C5 (3-hydroxyvalerate [3HV]) monomer units have modified physical properties; e.g., the plastic is less brittle than PHB, whereas PHAs containing C8 to C12 monomers behave as elastomers. This family of materials is the centre of considerable commercial interest, and 3HB-co-3HV copolymers have been marketed by ICI plc as Biopol. The known polymers exist as 2(1) helices with the fiber repeat decreasing from 0.596 nm for PHB to about 0.45 nm for C8 to C10 polymers. Novel copolymers with a backbone of 3HB and 4HB have been obtained. The native granules contain noncrystalline polymer, and water may possibly act as a plasticizer. Although the biosynthesis and regulation of PHB are generally well understood, the corresponding information for the synthesis of long-side-chain PHAs from alkanes, alcohols, and organic acids is still incomplete. The precise mechanisms of action of the polymerizing and depolymerizing enzymes also remain to be established. The structural genes for the three key enzymes of PHB synthesis from acetyl coenzyme A in Alcaligenes eutrophus have been cloned, sequenced, and expressed in Escherichia coli. Polymer molecular weights appear to be species specific. The factors influencing the commercial choice of organism, substrate, and isolation process are discussed. The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
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            Nucleic Acid Techniques in Bacterial Systematics

            A comprehensive laboratory manual written by specialists who have made significant contributions to developments of these techniques. Considers all of the major nucleic acid based techniques that are revolutionizing bacterial classification and identification. Also provides a comparison of methods for converting molecular data to phylogenetic trees.
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              Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements.

              Traditional mineral oil based plastics are important commodity to enhance the comfort and quality of life but the accumulation of these plastics in the environment has become a major universal problem due to their low biodegradation. Solution to the plastic waste management includes incineration, recycling and landfill disposal methods. These processes are very time consuming and expensive. Biopolymers are important alternatives to the petroleum-based plastics due to environment friendly manufacturing processes, biodegradability and biocompatibility. Therefore use of novel biopolymers, such as polylactide, polysaccharides, aliphatic polyesters and polyhydroxyalkanoates is of interest. PHAs are biodegradable polyesters of hydroxyalkanoates (HA) produced from renewable resources by using microorganisms as intracellular carbon and energy storage compounds. Even though PHAs are promising candidate for biodegradable polymers, however, the production cost limit their application on an industrial scale. This article provides an overview of various substrates, microorganisms for the economical production of PHAs and its copolymers. Recent advances in PHAs to reduce the cost and to improve the performance of PHAs have also been discussed.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                dyna
                DYNA
                Dyna rev.fac.nac.minas
                Universidad Nacional de Colombia (Medellín, Antioquia, Colombia )
                0012-7353
                March 2019
                : 86
                : 208
                : 75-81
                Affiliations
                [1] Medellín Antioquía orgnameUniversidad de Antioquia orgdiv1School of Microbiology orgdiv2Biotransformation Group Colombia wilman.alcaraz@ 123456udea.edu.co
                Article
                S0012-73532019000100075
                10.15446/dyna.v86n208.72019
                a4df67d7-b4c9-4c5b-b509-ee3128690bda

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 03 May 2018
                : 19 December 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 50, Pages: 7
                Product

                SciELO Colombia

                Categories
                Articles

                polihidroxialcanoatos,Ralstonia eutropha,Burkholderia sp.,hidrolizados de harina de yuca,biopolymers,polyhydroxyalkanoates,cassava flour hydrolysates,biopolímeros

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