Sialoglycoproteins Isolated from the Eggs of Gadus morhua Inhibit Bone Resorption in Ovariectomized Rats by Suppressing the MAPK and NF-κB Pathways

Abstract

The present study investigated the anti-osteoporosis function and the mechanism of sialoglycoproteins isolated from the eggs of Gadus morhua ( Gm-SGP) on ovariectomized (OVX) rats. After 3 months of Gm-SGP treatment, OVX-induced bone loss was suppressed and uncoupling bone turnover was balanced, as indicated by systemic biomarkers of bone metabolism; no uterine estrogenicity was observed. Moreover, rats administered with Gm-SGP exhibited increased bone mineral density and biomechanical strength and significant restoration of the trabecular microarchitecture compared with rats in the control group. Gm-SGP significantly decreased bone resorption-related indicators in serum. Investigation of the associated mechanisms revealed that Gm-SGP significantly increases the OPG/RANKL ratio at the mRNA and protein levels. Further research suggested that Gm-SGP inhibits the mRNA and protein expressions of important transcription factors of the MAPK and NF-κB signaling pathways. It also attenuates the activation of related transduction signaling pathways by inhibiting phosphorylation of JNK, ERK, p38, and NF-κB, and ultimately suppresses the induction of c-Fos and NFATc1. Overall, these results demonstrate that Gm-SGP inhibits bone resorption by suppressing osteoclastogenesis-related MAPK and NF-κB pathways, thereby improving osteoporosis.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 24 August 2019

Publication date (Print): 01 October 2019

Volume: 18

Issue: 5

Pages: 1174-1184

Affiliations

¹College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

Author notes

*Corresponding authors: WANG Jingfeng and XUE Changhu

Article

Publisher ID: s11802-019-3881-y

DOI: 10.1007/s11802-019-3881-y

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802