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      Stimulatory Effects of Androgens on Normal Children’s Bone Marrow in Culture: Effects on BFU-E, CFU-E, and Uroporphyrinogen I Synthase Activity

      ,

      Hormone Research in Paediatrics

      S. Karger AG

      Uroporphyrinogen I synthase, CFU-E, BFU-E, Androgens

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          Abstract

          We studied the effect of natural and synthetic androgens on children’s erythropoietic precursor cells in culture. Cultures of normal marrow were carried out according to a miniaturized methylcellulose method in the presence of erythropoietin. We then evaluated the effects of testosterone, nortestosterone, fluoxymesterone and etiocholanolone (10<sup>-9</sup>–10<sup>-6</sup> M) on erythroid colony-forming units (CFU-E) and burst-forming units (BFU-E). Androgen-induced growth of erythroid progenitors was quantified by directly scoring colonies and by a biochemical determination of the uroporphyrinogen I synthase activity (UROS). We observed a significant increase (p ≤ 0.05) in the number of CFU-E and BFU-E and in the UROS activity of derived colonies in the presence of androgens (10<sup>-8</sup> or 10<sup>-7</sup> M). This microculture assay could be useful not only to study the effect of androgens on erythroid progenitor cells in culture, but also to predict the best androgenic treatment of anemia in children and adults.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1986
          1986
          28 November 2008
          : 23
          : 2
          : 91-98
          Affiliations
          Unité de Recherches sur la Biochimie des Stéroïdes INSERM U-58 (Prof. Crastes de Paulet), Laboratoire de Biochimie B (Prof. Magnan de Bornier), Département de Pédiatrie (Prof. Jean) et Laboratoire des Stéroïdes (Hôpital Lapeyronie), Montpellier, France
          Article
          180295 Horm Res 1986;23:91–98
          10.1159/000180295
          3943773
          © 1986 S. Karger AG, Basel

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          Page count
          Pages: 8
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