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      Precisely and accurately localizing single emitters in fluorescence microscopy.

      Nature Methods
      Fluorescent Dyes, chemistry, Microscopy, Fluorescence, methods, standards, Reproducibility of Results

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          Abstract

          Methods based on single-molecule localization and photophysics have brought nanoscale imaging with visible light into reach. This has enabled single-particle tracking applications for studying the dynamics of molecules and nanoparticles and contributed to the recent revolution in super-resolution localization microscopy techniques. Crucial to the optimization of such methods are the precision and accuracy with which single fluorophores and nanoparticles can be localized. We present a lucid synthesis of the developments on this localization precision and accuracy and their practical implications in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.

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          Author and article information

          Journal
          24577276
          10.1038/nmeth.2843

          Chemistry
          Fluorescent Dyes,chemistry,Microscopy, Fluorescence,methods,standards,Reproducibility of Results

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