24
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma.

      Nature
      Amino Acid Sequence, Animals, Base Sequence, CCAAT-Enhancer-Binding Proteins, Cell Line, Chromosomes, Human, Pair 12, Chromosomes, Human, Pair 16, Cloning, Molecular, DNA, Neoplasm, DNA-Binding Proteins, genetics, Escherichia coli, HeLa Cells, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Liposarcoma, Mice, Molecular Sequence Data, Neoplasm Proteins, Nuclear Proteins, Protein Multimerization, RNA-Binding Protein EWS, RNA-Binding Protein FUS, RNA-Binding Proteins, Ribonucleoproteins, Sequence Homology, Amino Acid, Transcription Factor CHOP, Transcription Factors, Transfection, Translocation, Genetic, Tumor Cells, Cultured

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Human myxoid liposarcomas contain a characteristic chromosomal translocation, t(12;16)(q13;p11), that is associated with a structural rearrangement of the gene encoding CHOP, a growth arrest and DNA-damage inducible member of the C/EBP family of transcription factors residing on 12q13.1. Using a CHOP-specific complementary probe and antiserum we report here the presence of an abnormal CHOP transcript and protein in these tumours. Cloning of the translocation-associated CHOP gene product revealed a fusion between CHOP and a gene provisionally named TLS (translocated in liposarcoma). TLS is a novel nuclear RNA-binding protein with extensive sequence similarity to EWS, the product of a gene commonly translocated in Ewing's sarcoma. In TLS-CHOP the RNA-binding domain of TLS is replaced by the DNA-binding and leucine zipper dimerization domain of CHOP. Targeting of a conserved effector domain of RNA-binding proteins to DNA may play a role in tumour formation.

          Related collections

          Author and article information

          Comments

          Comment on this article