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      Proposal ofBurkholderiagen. nov. and Transfer of Seven Species of the GenusPseudomonasHomology Group II to the New Genus, with the Type SpeciesBurkholderia cepacia(Palleroni and Holmes 1981) comb. nov.

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          Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

          A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.
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            Determination of DNA base composition by reversed-phase high-performance liquid chromatography

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              The phylogeny of purple bacteria: The beta subdivision

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                Author and article information

                Journal
                Microbiology and Immunology
                Microbiology and Immunology
                Wiley-Blackwell
                03855600
                December 1992
                December 1992
                : 36
                : 12
                : 1251-1275
                Article
                10.1111/j.1348-0421.1992.tb02129.x
                1283774
                a646942a-6c8d-4136-a17a-080a1c5ae042
                © 1992

                http://doi.wiley.com/10.1002/tdm_license_1.1

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