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      Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities

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          ABSTRACT

          mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

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          Author and article information

          Journal
          Applied and Environmental Microbiology
          AEM
          American Society for Microbiology
          0099-2240
          1098-5336
          December 01 2009
          December 01 2009
          October 02 2009
          : 75
          : 23
          : 7537-7541
          Affiliations
          [1 ] Department of Microbiology, University of Massachusetts, Amherst, Massachusetts
          [2 ] Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan
          [3 ] Department of Biology, University of New Mexico, Albuquerque, New Mexico
          [4 ] Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
          [5 ] Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas
          [6 ] Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota
          [7 ] Department of Biological Sciences, University of Warwick, Coventry, United Kingdom
          [8 ] Faculty of Computer Science, Dalhousie University, Halifax, NS, Canada
          [9 ] Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado
          [10 ] Department of Animal Science, University of Ljubljana, Ljubljana, Slovenia
          [11 ] Institute for Genomics and Bioinformatics, Graz University of Technology, Graz, Austria
          [12 ] Department of Biological Sciences, Louisiana State University, Baton Rouge, Lousiana
          Article
          10.1128/AEM.01541-09
          2786419
          19801464
          a67218cc-d69c-4767-9671-ddf874ecfb58
          © 2009
          Product
          Self URI (article page): https://AEM.asm.org/content/75/23/7537

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