For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
18
views
0
references
 Top references
cited by
0
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      237
      similar
       All similar
      Are you tired of sifting through news that doesn't interest you?
      Personalize your Karger newsletter today and get only the news that matters to you!

      Sign up

      • Record: found
      • Abstract: found
      • Article: found

      Lens Hexosaminidases: Purification and Properties

      research-article
      Author(s): ,
      Publication date (Electronic):
      Journal: Ophthalmic Research
      Publisher: S. Karger AG
      Keywords: pH, Kinetic constants, Rabbit lens, Capsule-epithelium, Hexosaminidase, Galactosamidase, Column chromatography

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Lens hexosaminidase(s), N-acetyl-β-D-glucosaminidase and N-acetyl-β-D-galactosaminidase, were purified from rabbit lens capsule-epithelium by centrifugation, dialysis and column chromatography on DEAE-cellulose ans Sephadex G-200. pH distribution curves and kinetic constants (K<sub>m</sub> and V<sub>max</sub>) were determined in the fractions purified approximately 700-fold. Lens glucosaminidase and galactosaminidase could not be separated from each other, thus indicating that one enzyme was present but unable to distinguish the C<sub>4</sub> position of aminosugars.

          Related collections

          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1976
          Publication date (Print): 1976
          Publication date (Electronic): 03 December 2009
          Volume: 8
          Issue: 1
          Pages: 25-33
          Affiliations
          Biochemical Laboratories, Department of Ophthalmology, University of Illinois Eye and Ear Infirmary, University of Illinois at the Medical Center, Chicago, Ill.
          Article
          Publisher ID: 264796 Other ID: Ophthalmic Res 1976;8:25–33
          DOI: 10.1159/000264796
          SO-VID: a69743a1-8c65-4ec8-bd33-cfcd2ecc5c95
          Copyright © © 1976 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 24 September 1974
          Date accepted : 03 February 1975
          Page count
          Pages: 9
          Categories
          Subject: Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: Galactosamidase,pH,Capsule-epithelium,Hexosaminidase,Column chromatography,Kinetic constants,Rabbit lens
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: Galactosamidase, pH, Capsule-epithelium, Hexosaminidase, Column chromatography, Kinetic constants, Rabbit lens

          Comments

          Comment on this article