The unequal variance signal detection (UVSD) model of recognition memory assumes that the variance of old item memory strength (σ o) is typically greater than that of new items. It has been suggested that this old item variance effect can be explained by the encoding variability hypothesis. However, Spanton and Berry (2020) failed to find evidence for this account, suggesting that σ o may simply scale with mean memory strength ( d) in the UVSD model. Experiments 1 and 2 examined the effects of encoding variability and strength scaling on old item variance by creating conditions in which mean memory strength and variability in item characteristics was either low or high in 2 × 2 factorial designs. In Experiment 1, overall strength determined estimates of σ o, with no effect of item characteristic variability. The same effect of overall strength was found in Experiment 2; there was also a significant effect of item characteristic variability, although this manipulation also had some effect on d and was therefore partially confounded. Experiment 3 similarly found a simultaneous increase in old item variance and memory strength in a design using mixed item characteristic variability conditions in a single-study/test block. We conclude that old item variance increases with mean memory strength in the UVSD model, with uncertainty about the effects of encoding variability, and that future explanations of the old item variance effect should bear this in mind.