4
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Hydroxyproline-containing collagen peptide derived from the skin of the Alaska pollack inhibits HIV-1 infection

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The human immunodeficiency virus (HIV) is a lentivirus that results in acquired immunodeficiency syndrome (AIDS). HIV treatment involving chemical therapeutic agents has improved the quality of life of HIV/AIDS patients. The present study demonstrates that a hydroxyproline-containing marine collagen peptide (APHCP) derived from Alaska pollack inhibits HIV-1 infection in the MT-4 human T cell-line. APHCP inhibited HIV-1 IIIB-induced cell lysis, syncytia formation, reverse transcriptase activity and viral p24 production at non-cytotoxic concentrations; however, APHCP did not inhibit HIV-2 ROD infection in MT-4 cells. This suggests that the anti-HIV activity of APHCP is specific to HIV-1. In addition, substitution of hydroxyproline residues in APHCP with prolines impaired its anti-HIV-1 activity, suggesting that the hydroxyl group of hydroxyprolines is required for the anti-HIV-1 activity of APHCP. These results suggested that the marine peptide APHCP may be a novel drug candidate in the development of next-generation therapeutic agents for the treatment of HIV/AIDS.

          Related collections

          Most cited references20

          • Record: found
          • Abstract: found
          • Article: not found

          Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin.

          Gelatin extracted from Alaska pollack skin was hydrolyzed with serial digestions in the order of Alcalase, Pronase E, and collagenase using a three-step recycling membrane reactor. The fraction from the second step, which was hydrolyzed with Pronase E, was composed of peptides ranging from 1.5 to 4.5 kDa and showed high antioxidative activity. Two different peptides showing strong antioxidative activity were isolated from the hydrolysate using consecutive chromatographic methods including gel filtration on a Sephadex G-25 column, ion-exchange chromatography on a SP-Sephadex C-25 column, and high-performance liquid chromatography on an ODS column. The isolated peptides, P1 and P2, were composed of 13 and 16 amino acid residues, respectively; and both peptides contained a Gly residue at the C-terminus and the repeating motif Gly-Pro-Hyp. The antioxidative activities of the purified peptides were measured using the thiobarbituric acid method, and the cell viability was measured with MTT assay. The results showed that P2 had potent antioxidative activity on peroxidation of linoleic acid. Moreover, the cell viability of cultured liver cells was significantly enhanced by addition of the peptide. These results indicate that the purified peptide, P2, from gelatin hydrolysate of Alaska pollack skin is a natural antioxidant which has potent antioxidative activity.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Anti-HIV-1 activity of phloroglucinol derivative, 6,6'-bieckol, from Ecklonia cava.

            Ecklonia cava (EC), which is an edible marine brown alga with a broad range of bioactivities, belongs to the family of Laminariaceae. The bioactive 6,6'-bieckol, one of the main phloroglucinol derivatives naturally occurred from this genus, was isolated and characterized by NMR techniques. For the first time, human immunodeficiency virus type-1 (HIV-1) inhibitory activity of 6,6'-bieckol showed wild inhibition against HIV-1 induced syncytia formation (EC(50) 1.72 microM), lytic effects (EC(50) 1.23 microM), and viral p24 antigen production (EC(50) 1.26 microM), respectively. This result was strongly and clearly supported by the further investigation also, which 6,6'-bieckol selectively inhibited the activity of HIV-1 reverse transcriptase (RT) enzyme with EC(50) of 1.07 microM, as well as HIV-1 entry. Moreover, unlike most of other tannins, 6,6'-bieckol exhibited no cytotoxicity at concentrations which inhibited HIV-1 replication almost completely. Thus, it can be suggested that the potentially effective 6,6'-bieckol might be employed as a drug candidate for development of new generation therapeutic agents against HIV.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Isolation of human T-cell leukemia virus in acquired immune deficiency syndrome (AIDS).

              Several isolates of a human type-C retrovirus belonging to one group, known as human T-cell leukemia virus (HTLV), have previously been obtained from patients with adult T-cell leukemia or lymphoma. The T-cell tropism of HTLV and its prevalence in the Caribbean basin prompted a search for it in patients with the epidemic T-cell immune deficiency disorder known as AIDS. Peripheral blood lymphocytes from one patient in the United States and two in France were cultured with T-cell growth factor (TCGF) an shown to express HTLV antigens. Virus from the U.S. patient was isolated and characterized and shown to be related to HTLV subgroup I. The virus was also transmitted into normal human T cells from umbilical cord blood of a newborn. Whether or not HTLV-I or other retroviruses of this family with T-cell tropism cause AIDS, it is possible that patients from whom the virus can be isolated can also transmit it to others. If the target cell of AIDS is the mature T cell as suspected, the methods used in these studies may prove useful for the long-term growth of these cells and for the identification of antigens specific for the etiological agent of AIDS.
                Bookmark

                Author and article information

                Journal
                Mol Med Rep
                Mol Med Rep
                Molecular Medicine Reports
                D.A. Spandidos
                1791-2997
                1791-3004
                December 2016
                16 November 2016
                16 November 2016
                : 14
                : 6
                : 5489-5494
                Affiliations
                Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea
                Author notes
                Correspondence to: Professor Sun Joo Park, Department of Chemistry, Pukyong National University, 599-1 Daeyeon 3-Dong, Nam-Gu, Busan 608-737, Republic of Korea, E-mail: parksj@ 123456pknu.ac.kr
                Article
                mmr-14-06-5489
                10.3892/mmr.2016.5949
                5355660
                27878297
                a6a0b0f6-212e-47b3-80f0-57ca71e268bd
                Copyright: © Jang et al.

                This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

                History
                : 23 October 2015
                : 13 October 2016
                Categories
                Articles

                human immunodeficiency virus,marine,acquired immunodeficiency syndrome,peptide,activity

                Comments

                Comment on this article