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      Highly efficient RNA-guided base editing in mouse embryos

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      Nature Biotechnology

      Springer Nature

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          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Mice with targeted point mutations are generated efficiently using Cas9–cytidine deaminase fusions.

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          Most cited references 4

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          Knockout mice created by TALEN-mediated gene targeting.

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            Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.

            RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F₀ and F₁ mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research.
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              Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins.

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                Author and article information

                Journal
                Nature Biotechnology
                Nat Biotechnol
                Springer Nature
                1087-0156
                1546-1696
                February 27 2017
                February 27 2017
                :
                :
                Article
                10.1038/nbt.3816
                28244995
                © 2017
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