For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
47
views
4
references
 Top references
cited by
190
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      0
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: not found

      Highly efficient RNA-guided base editing in mouse embryos

      Author(s): , , , , , , , ,
      Publication date Created:
      Publication date (Print):
      Journal: Nature Biotechnology
      Publisher: Springer Nature

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Mice with targeted point mutations are generated efficiently using Cas9–cytidine deaminase fusions.

          Related collections

          Most cited references4

          • Record: found
          • Abstract: not found
          • Article: not found

          Knockout mice created by TALEN-mediated gene targeting.

          Jaehoon Lee, Hye Jeon, Byoung Kim … (2012)
          Article 0 comments Cited 135 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

            Young Hoon Sung, Jong Kim, Hyun-Taek Kim … (2014)
            RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F 0 and F 1 mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research.
            Article 0 comments Cited 118 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins.

              Junho K Hur, Kyoungmi Kim, Kyung Wook Been … (2016)
              Article 0 comments Cited 97 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Title: Nature Biotechnology
                Abbreviated Title: Nat Biotechnol
                Publisher: Springer Nature
                ISSN (Print): 1087-0156
                ISSN (Electronic): 1546-1696
                Publication date Created: February 27 2017
                Publication date (Print): February 27 2017
                Volume:
                Issue:
                Article
                DOI: 10.1038/nbt.3816
                PubMed ID: 28244995
                SO-VID: a6c1e9d9-82b3-4007-b554-9de4804a475d
                Copyright © © 2017
                History

                Data availability:

                Comments

                Comment on this article