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      Developmental Change in Na,K-ATPase α1 and β1 Expression in Normal and Hypothyroid Rat Renal Cortex

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          Abstract

          Renal Na,K-ATPase drives active reabsorption of sodium and cotransported solutes along the nephron. There is a large increase in net Na<sup>+</sup> reabsorption in the postnatal rat kidney. It has previously been established that the postnatal increase in expression of sodium pump isoforms in the brain, but not the heart, is blunted in the hypothyroid neonate. The aims of this study were to establish whether the developmental increase in renal sodium transport is associated with coordinate increases in the abundance of the sodium pump α<sub>1</sub> catalytic and β<sub>1</sub> glycoprotein subunits and Na,K-ATPase activity, and to determine whether thyroid status influences the postnatal increase in renal Na,K-ATPase expression. Pregnant rats were made hypothyroid with low iodine diet, propylthiouracil and perchlorate. Offspring were hypothyroid assessed by triiodothyronine/thyroxine RIA. Renal cortical membranes were prepared from euthyroid and hypothyroid rats from 6 to 24 days of age. There was no change in Na,K-ATPase activity or expression between 6 and 15 days. Between 15 and 24 days, Na,K-ATPase activity increased 1.35-fold while sodium pump α<sub>1</sub> and β<sub>1</sub> subunit abundance increased coordinately to 1.7- and 2-fold over the previous period, respectively. In hypothyroid neonates, kidney weight was less than in euthyroids, and Na,K-ATPase activity, α<sub>1</sub> and β<sub>1</sub> subunit pool sizes did not significantly increase as a function of age between 6 and 24 days. We conclude that the postnatal increase in sodium pump activity can be accounted for by coordinate increases in the pool sizes of α<sub>1</sub> and β<sub>1</sub> subunits and that, like in brain, this increased Na,K-ATPase expression is dependent on normal thyroid status.

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          Author and article information

          Journal
          AJN
          Am J Nephrol
          10.1159/issn.0250-8095
          American Journal of Nephrology
          S. Karger AG
          0250-8095
          1421-9670
          2000
          June 2000
          30 June 2000
          : 20
          : 3
          : 225-231
          Affiliations
          aDepartment of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles, Calif., USA; bDepartment of Nephrology, Rambam Medical Center, Haifa, Israel
          Article
          13593 Am J Nephrol 2000;20:225–231
          10.1159/000013593
          10878407
          © 2000 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Figures: 4, References: 28, Pages: 7
          Product
          Self URI (application/pdf): https://www.karger.com/Article/Pdf/13593
          Categories
          Laboratory Investigation

          Cardiovascular Medicine, Nephrology

          Sodium pump, Kidney, Development, Na,K-ATPase, Thyroid hormone

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