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      Comparative Chromatography of Hypothalamic Corticotrophin-Releasing Factors

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          Abstract

          Extracts of rat stalk-median eminences were chromatographed on Sephadex G-50 and BioGel P2. Effluent fractions were monitored for corticotrophin-releasing factor (CRF) bioactivity, using the perfused isolated pituitary cell column bioassay, and radioimmunoassay for CRF-41, AVP, oxytocin, neurophysin, adrenocorticotrophic hormone (ACTH) and luteinizing hormone-releasing hormone (LHRH). Porcine hypothalami were also chromatographed on Sephadex G-50. Our results suggest that vasopressin and a peptide in the rat related to synthetic ovine CRF are two components of the hypothalamic CRF complex which potentiate each other at the corticotrope to release ACTH. However, these two substances alone do not appear to be wholly responsible for CRF bioactivity of hypothalamic stalk-median eminence extracts and other CRFs may yet be found.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1984
          1984
          28 March 2008
          : 38
          : 1
          : 17-24
          Affiliations
          Protein Hormone and Recombiant DNA Unit, St. Bartholomew’s Centre for Clinical Research, London, UK
          Article
          123860 Neuroendocrinology 1984;38:17–24
          10.1159/000123860
          6607424
          © 1984 S. Karger AG, Basel

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          Page count
          Pages: 8
          Categories
          Original Paper

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