Comparative Chromatography of Hypothalamic Corticotrophin-Releasing Factors

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Extracts of rat stalk-median eminences were chromatographed on Sephadex G-50 and BioGel P2. Effluent fractions were monitored for corticotrophin-releasing factor (CRF) bioactivity, using the perfused isolated pituitary cell column bioassay, and radioimmunoassay for CRF-41, AVP, oxytocin, neurophysin, adrenocorticotrophic hormone (ACTH) and luteinizing hormone-releasing hormone (LHRH). Porcine hypothalami were also chromatographed on Sephadex G-50. Our results suggest that vasopressin and a peptide in the rat related to synthetic ovine CRF are two components of the hypothalamic CRF complex which potentiate each other at the corticotrope to release ACTH. However, these two substances alone do not appear to be wholly responsible for CRF bioactivity of hypothalamic stalk-median eminence extracts and other CRFs may yet be found.

Related collections

Author and article information

Journal

Journal ID (publisher-id): NEN

Journal ID (nlm-ta): Neuroendocrinology

Journal ID (doi): 10.1159/issn.0028-3835

Title: Neuroendocrinology

Publisher: S. Karger AG

ISSN (Print): 0028-3835

ISSN (Electronic): 1423-0194

Publication date Subscription-year: 1984

Publication date (Print): 1984

Publication date (Electronic): 28 March 2008

Volume: 38

Issue: 1

Pages: 17-24

Affiliations

Protein Hormone and Recombiant DNA Unit, St. Bartholomew’s Centre for Clinical Research, London, UK

Article

Publisher ID: 123860 Other ID: Neuroendocrinology 1984;38:17–24

DOI: 10.1159/000123860

PubMed ID: 6607424

SO-VID: a7206c6e-bdec-4ea6-9bcd-9fecb30224ca

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 07 February 1983

Date accepted : 09 June 1983

Page count

Pages: 8

Comments

Comment on this article

scite_