6
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          After sperm-oocyte fusion, cortical granules (CGs) located in oocyte cortex undergo exocytosis and their content is released into the perivitelline space to avoid polyspermy. Thus, cortical granule exocytosis (CGE) is a key process for fertilization success. We have demonstrated that alpha-SNAP -and its functional partner NSF- mediate fusion of CGs with the plasma membrane in mouse oocytes. Here, we examined at cellular and ultrastructural level oocytes from hyh (hydrocephalus with hop gait) mice, which present a missense mutation in the Napa gene that results in the substitution of methionine for isoleucine at position 105 (M105I) of alpha-SNAP. Mutated alpha-SNAP was mislocalized in hyh oocytes while NSF expression increased during oocyte maturation. Staining of CGs showed that 9.8% of hyh oocytes had abnormal localization of CGs and oval shape. Functional tests showed that CGE was impaired in hyh oocytes. Interestingly, in vitro fertilization assays showed a decreased fertilization rate for hyh oocytes. Furthermore, fertilized hyh oocytes presented an increased polyspermy rate compared to wild type ones. At ultrastructural level, hyh oocytes showed small mitochondria and a striking accumulation and secretion of degradative structures. Our findings demonstrate the negative effects of alpha-SNAP M105 mutation on oocyte biology and further confirm the relevance of alpha-SNAP in female fertility.

          Related collections

          Most cited references73

          • Record: found
          • Abstract: found
          • Article: not found

          The road to maturation: somatic cell interaction and self-organization of the mammalian oocyte.

          Mammalian oocytes go through a long and complex developmental process while acquiring the competencies that are required for fertilization and embryogenesis. Recent advances in molecular genetics and quantitative live imaging reveal new insights into the molecular basis of the communication between the oocyte and ovarian somatic cells as well as the dynamic cytoskeleton-based events that drive each step along the pathway to maturity. Whereas self-organization of microtubules and motor proteins direct meiotic spindle assembly for achieving genome reduction, actin filaments are instrumental for spindle positioning and the establishment of oocyte polarity needed for extrusion of polar bodies. Meiotic chromatin provides key instructive signals while being 'chauffeured' by both cytoskeletal systems.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            SNARE proteins are required for macroautophagy.

            Macroautophagy mediates the degradation of long-lived proteins and organelles via the de novo formation of double-membrane autophagosomes that sequester cytoplasm and deliver it to the vacuole/lysosome; however, relatively little is known about autophagosome biogenesis. Atg8, a phosphatidylethanolamine-conjugated protein, was previously proposed to function in autophagosome membrane expansion, based on the observation that it mediates liposome tethering and hemifusion in vitro. We show here that with physiological concentrations of phosphatidylethanolamine, Atg8 does not act as a fusogen. Rather, we provide evidence for the involvement of exocytic Q/t-SNAREs in autophagosome formation, acting in the recruitment of key autophagy components to the site of autophagosome formation, and in regulating the organization of Atg9 into tubulovesicular clusters. Additionally, we found that the endosomal Q/t-SNARE Tlg2 and the R/v-SNAREs Sec22 and Ykt6 interact with Sso1-Sec9, and are required for normal Atg9 transport. Thus, multiple SNARE-mediated fusion events are likely to be involved in autophagosome biogenesis. Copyright © 2011 Elsevier Inc. All rights reserved.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Oocyte control of ovarian follicular development and function in mammals.

              A new perspective on ovarian follicular development has emerged over the last decade. Whereas the oocyte was previously considered only a passive recipient of developmental signals from oocyte-associated granulosa cells, it is now clear that communication between oocytes and granulosa cells is bidirectional. A complex interplay of regulatory factors governs the development of both types of cell. This interplay is essential not only for oocyte development but also for follicular development, beginning with the initial assembly of the primordial follicle and continuing throughout ovulation. The existence of an oocyte-granulosa cell regulatory loop, essential for normal follicular differentiation as well as for the production of an oocyte competent to undergo fertilization and embryogenesis, is proposed. Although gonadotrophins are essential for driving the differentiation of granulosa cell phenotypes, within its sphere of influence, the oocyte is probably the dominant factor determining the direction of differentiation and the function of the granulosa cells associated with it.
                Bookmark

                Author and article information

                Contributors
                lbatiz@uandes.cl
                mmichaut@gmail.com
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                22 November 2019
                22 November 2019
                2019
                : 9
                : 17374
                Affiliations
                [1 ]ISNI 0000 0001 2185 5065, GRID grid.412108.e, Instituto de Histología y Embriología, Universidad Nacional de Cuyo-CONICET, ; Mendoza, Argentina
                [2 ]ISNI 0000 0001 2185 5065, GRID grid.412108.e, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, ; Mendoza, Argentina
                [3 ]ISNI 0000 0001 2185 5065, GRID grid.412108.e, Instituto de Medicina y Biología Experimental de Cuyo, Universidad Nacional de Cuyo-CONICET, ; Mendoza, Argentina
                [4 ]ISNI 0000 0004 0487 459X, GRID grid.7119.e, Instituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, ; Valdivia, Chile
                [5 ]ISNI 0000 0004 0487 459X, GRID grid.7119.e, Instituto de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, ; Valdivia, Chile
                [6 ]ISNI 0000 0004 0487 6659, GRID grid.440627.3, Centro de Investigación e Innovación Biomédica (CIIB), Facultad de Medicina, Universidad de los Andes, ; Santiago, Chile
                [7 ]ISNI 0000 0001 2185 5065, GRID grid.412108.e, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, ; Mendoza, Argentina
                Author information
                http://orcid.org/0000-0002-0528-6605
                Article
                53574
                10.1038/s41598-019-53574-8
                6874563
                31758001
                a796f333-f4ae-4bf4-a24b-7597e2f0f652
                © The Author(s) 2019

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 10 July 2019
                : 30 October 2019
                Funding
                Funded by: PLISSER
                Funded by: Chilean FONDECYT Regular grants 1141015
                Categories
                Article
                Custom metadata
                © The Author(s) 2019

                Uncategorized
                cell biology,medical research
                Uncategorized
                cell biology, medical research

                Comments

                Comment on this article