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      OCCURRENCY OF MALASSEZIA PACHYDERMATIS AND OTHER INFECTIOUS AGENTS AS CAUSE OF EXTERNAL OTITIS IN DOGS FROM RIO GRANDE DO SUL STATE, BRAZIL (1996/1997) Translated title: Ocorrência de Malassezia pachydermatis e de outros agentes etiológicos de otite externa em cães no estado do Rio Grande do Sul, BR (1996-1997)

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          Abstract

          The purpose of this work was to evaluate the frequency of Malassezia pachydermatis infection and other infectious agents in dogs with external otitis and with healthy auditory tubes. Clinical manifestations of external otitis were evaluated. Samples from the auditory tube of 102 dogs with otitis and from 32 healthy dogs were submitted to direct microscopic examination and cultured in blood agar and Sabouraud dextrose agar with chloramphenicol and cycloheximide. Direct examination showed more than ten cells of M. pachydermatis in 52.0% of the samples from dogs with otitis, but in only 21.8% of the healthy auditory tube samples. M. pachydermatis was isolated in 37.5% of the samples from dogs with healthy auditory tube and 76.5% (p<0.01) of the samples from dogs with otitis. There was an association between M. pachydermatis and Staphylococcus aureus (p<0.01), but not with Pseudomonas aeruginosa (p>0.05). Infection by M. pachydermatis was prevalent in the following breeds: Cocker Spainel, German Shepherd and Brazilian Fila. No differences were found in frequency of the infection in relation to age, sex and ear anatomy of the dogs. Otomycosis were predominantly ceruminous and erythematous. M. pachydermatis was the most frequent agent in external otitis.

          Translated abstract

          Este trabalho teve como objetivos estudar a ocorrência da Malassezia pachydermatis e de outros agentes etiológicos em cães com otite externa e com conduto auditivo externo hígido; caracterizar a otomicose por M. pachydermatis. Foram analisadas 32 amostras de cães com o conduto auditivo hígido e 102 de otite externa. Todas as amostras foram submetidas ao exame direto, cultivo em ágar sangue e em ágar Sabouraud dextrose acrescido de cloranfenicol e cicloheximida. Ao exame direto 52.0% das amostras de otite apresentaram mais de dez células de M. pachydermatis. Somente 21.8% das amostras de orelhas hígidas apresentaram no exame direto células com esta morfologia (uma a dez células). M. pachydermatis foi isolada em 37.5% das amostras do conduto auditivo hígido e em 76.5% das amostras de otite (p<0.01). Foi freqüente a associação entre Malassezia pachydermatis e Staphylococcus aureus (p<0.01) enquanto a associação da levedura com Pseudomonas aeruginosa não foi comum (p>0.05). A infecção por M. pachydermatis foi prevalente em cães da raça Cocker Spainel, Pastor Alemão e Fila Brasileiro, não tendo sido demonstradas diferenças para idade, sexo e conformação do pavilhão auricular. A otomicose foi predominantemente ceruminosa e eritematosa. M. pachydermatis foi o agente com maior prevalência nas otites externas.

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          Most cited references21

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          Characterization of markedly lipid-dependent Malassezia pachydermatis isolates from healthy dogs.

          When 244 Malassezia colonies which had been isolated from a colony of Beagle dogs using modified Dixon's agar were sub-cultured on Sabouraud's dextrose agar to determine their lipid dependence, 30 showed poor growth resembling M. furfur, whereas the remainder were typical of M. pachydermatis. Eight of the 10 poor growing isolates selected for further study formed colonies typical of M. pachydermatis after five passages on Sabouraud's dextrose agar at 4 d intervals and two continued to show poor growth. Nine isolates had enzyme profiles identical to those of typical M. pachydermatis isolates, and one resembled M. furfur. However, seven of the poor growing isolates which were karyotyped had patterns typical of M. pachydermatis. Poor growing isolates and their non-lipid-dependent 'revertants' had identical restriction fragment length polymorphism patterns and poly(GT) hybridization profiles. These observations show that some M. pachydermatis isolates grow poorly when sub-cultured onto Sabouraud's dextrose agar and may be incorrectly identified as M. furfur if further studies are not performed.
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            Newly reported skin disease syndromes in the dog.

            Several recently recognized canine dermatoses are described. Syndromes discussed include superficial necrolytic dermatitis, Malasezzia dermatitis, sebaceous adenitis, nodular dermatofibrosis in German shepherd dogs, solar-induced dermatitis, and acrodermatitis.
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              Dermatitis in dogs caused by Malassezia (Pityrosporum) pachydermatis

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjm
                Brazilian Journal of Microbiology
                Braz. J. Microbiol.
                Sociedade Brasileira de Microbiologia (São Paulo )
                1678-4405
                October 2001
                : 32
                : 3
                : 245-249
                Affiliations
                [1 ] Universidade Federal de Pelotas Brazil
                [2 ] Universidade Federal do Rio Grande do Sul Brasil
                Article
                S1517-83822001000300017
                10.1590/S1517-83822001000300017
                a7ea6e4f-d0af-42cb-ae19-08791e0e3b78

                http://creativecommons.org/licenses/by/4.0/

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                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382&lng=en
                Categories
                MICROBIOLOGY

                Microbiology & Virology
                Malassezia pachydermatis,Staphylococcus aureus,otitis,dog,otites,cães
                Microbiology & Virology
                Malassezia pachydermatis, Staphylococcus aureus, otitis, dog, otites, cães

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